一、基本信息
李爱涛，博士，教授，博导，省部共建生物催化与酶工程国家重点实验室副主任
电子邮箱：aitaoli(at)hubu.edu.cn; aitaoli(at)163.com
学术兼职：《Bioresources and Bioprocessing》和《Biodesign Research》副主编，Green Synthesis and Catalysis》、《Bioresources and Bioprocessing》青年编委，《Applied Biochemistry and Biotechnology》编委。中国微生物学会酶工程专业委员会委员，中国生物化工青年委员会委员，湖北省生物催化与酶工程技术引智示范基地负责人，湖北省微生物学会理事，湖北省合成生物学学会理事
研究方向：酶定向进化方法学的开发（计算机辅助的精准设计、机器学习的酶分子改造）；含亚铁血红素Heme氧化还原酶的酶学及酶工程（P450酶及UPO酶元件创制与改造）；细胞色素P450在医药、天然产物生物合成中的应用（人工生物合成途径的创建）
二、教育背景
2005–2010 华东理工大学生物反应器工程国家重点实验室，博士学位
2001–2005 河北工业大学，生物工程系本科生，学士学位
三、工作经历
2017至今 湖北大学生命科学学院，教授
2014–2017 德国马普所（煤炭化学研究所），博士后研究员
2011–2014 新加坡国立大学化学与生物分子工程系，研究员
四、科研项目
1. 科技部“合成生物学”重点研发计划课题项目，2019YFA0905002，人工途径生物酶元件的理性设计与结构改造，2020-1至2024-12，500万元，在研，主持
2. 科技部重点研发计划子课题项目，2021YFC2102703, 多酶体系中自然和人工辅因子的再生与调控策略，2021-1至2024-11，63万元，在研
3. 国家自然科学基金委员会，面上项目，21977026，新型P450甾体羟化酶的分子调控机制及其理性改造，2020-01至 2023-12，66万元，在研，主持
4. 湖北省科技厅外专处，引智基地项目，XKCX2019000002，生物催化与酶工程技术， 2020-01至今，110万元，主持，在研
5. 国家自然科学基金委员会，青年科学基金项目，21702052，基于P450酶定向羟化的去甲（伪）麻黄碱对映体新合成途径研究，2018-01至 2020-12，26万元，在研，主持
五、代表性论文（IF>10；#共同第一作者，*通讯作者）
1. Zili Zhang, Chenghua Gao, Jing Zhao, Xiao-Gang Peng, Qian Li, Aitao Li*. A designed chemoenzymatic route for efficient synthesis of 6-dehydronandrolone acetate: a key precursor in the synthesis of C7-functionalized steroidal drugs. ACS Catalysis 2023, 13, 13111–13116.
2. Zhongwei Zhang#, Lin Fang#, Fei Wang, Yu Deng, Zhengbin Jiang, Aitao Li*. Transforming Inert Cycloalkanes into α,ω-Diamines by DesignedEnzymatic Cascade Catalysis. Angew. Chem. Int. Ed. 2023, e202215935.
3. Ruiwen Hu#, Anjie Gong#, Langxing Liao#, Yan‐Xin Zheng#, Xin Liu, Peng Wu, Fushuai Li, Huili Yu, Jing Zhao, Long-Wu Ye*, Binju Wang*, Aitao Li*. Biocatalytic aminohydroxylation of styrenes for efficient synthesis of enantiopure β-amino alcohols. Chinese Journal of Catalysis. 2023, 44, 171–178.
4. Aitao Li, Qian Wang, Xitong Song, Xiaodong Zhang, Jian-Wen Huang, Chun-Chi Chen, Rey-Ting Guo*, Binju Wang*, Manfred T. Reetz*. Engineering of a P450‐based Kemp eliminase with a new mechanism. Chinese Journal of Catalysis. 2023,47, 191–199.
5. Xiaodong Zhang, Panpan Shen, Jing Zhao, Yueyue Chen, Xian Li, Jian-Wen Huang, Lilan Zhang, Qian Li, Chenghua Gao, Qiong Xing, Chun-Chi Chen, Rey-Ting Guo,* and Aitao Li*. Rationally Controlling Selective Steroid Hydroxylation via Scaffold Sampling of a P450 Family.. ACS Catalysis, 2023, 13 (2), 1280–1289.
6. Yaqin Peng#, Chenghua Gao#, Zili Zhang, Shijie Wu, Jing Zhao*, Aitao Li.*. A Chemoenzymatic Strategy for the Synthesis of Steroid Drugs Enabled by P450 Monooxygenase-Mediated Steroidal Core Modification. ACS Catalysis, 2022, 12, 2907-2914.
7. Chun-Chi Chen#, Meng Dai#, Lilan Zhang#, Jing Zhao, Wei Zeng, Min Shi, Jian-Wen Huang, Weidong Liu, Rey-Ting Guo*, Aitao Li.*. Molecular Basis for a Toluene Monooxygenase to Govern Substrate Selectivity. ACS Catalysis, 2022, 12, 2831-2839.
8. Fei Wang#, Jing Zhao#, Qian Li#, Jun Yang, Renjie Li, Jian Min, Xiaojuan Yu, Gao-Wei Zheng, Hui-Lei Yu, Chao Zhai, Carlos G. Acevedo-Rocha, Lixin Ma, Aitao Li*. One-pot biocatalytic route from cycloalkanes to α, ω‐dicarboxylic acids by designed Escherichia coli consortia. Nature communications, 2020, 11, 5035.
9. Zhongwei Zhang#, Qian Li#, Fei Wang#, Ren-Jie Li, Xiaojuan Yu, Lixin Kang, Jing Zhao and Aitao Li.*. One-Pot Biosynthesis of 1, 6-Hexanediol from Cyclohexane by de novo Designed Cascade Biocatalysis. Green Chemistry, 2020, 2020, 22, 7476-7483.
10. Aitao Li,*# Carlos G Acevedo-Rocha,* # Lorenzo D’Amore,# Jinfeng Chen,# Yaqin Peng, Marc Garcia-Borràs, Chenghua Gao, Jinmei Zhu, Harry Rickerby, Sílvia Osuna*, Jiahai Zhou*, Manfred T Reetz*. Regio‐and Stereoselective Steroid Hydroxylation at the C7‐Position by Cytochrome P450 Monooxygenase Mutants. Angew. Chem. Int. Ed. 2020, 59, 12499–12505. (Hot paper, highlighted in Synfacts)
11. Hangyu Zhou,# Binju Wang,# Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li*, Manfred T. Reetz*. Chemo- and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase. Angew. Chem. Int. Ed. 2019, 58, 764–768 (Back cover).
12. Yucai He*, Yun Ding , Cuiluan Ma , Junhua Di , Chunxia Jiang and Aitao Li*. One-pot conversion of biomass-derived xylose to furfuralcohol by a chemo-enzymatic sequential acid-catalyzed dehydration and bioreduction. Green Chemistry, 2017, 19 (16), 3844-3850.
13. Ai-Tao Li#, Ge Qu#, Zhoutong Sun*, Manfred T Reetz*. Statistical analysis of the benefits of focused saturation mutagenesis in directed evolution based on reduced amino acid alphabets. ACS Catalysis, 2019, 9, 7769-7778.
14. Aitao Li,# Binju# Wang, Adriana Ilie, Kshatresh D. Dubey, Gert Bange, Ivan V. Korendovych, Sason Shaik*, Manfred T. Reetz*. A redox-mediated Kemp eliminase. Nature communications, 2017, 8, 14876.
15. Aitao Li, Adriana Ilie, Zhoutong Sun, Richard Lonsdale, Jian-He Xu, Manfred T. Reetz*. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. Angew. Chem. Int. Ed. 2016, 55, 128,12205-12208.
16. Carlos G. Acevedo-Rocha#, Aitao Li #, Lorenzo D’Amore#, Sabrina Hoebenreich, Joaquin Sanchis, Paul Lubrano, Matteo P. Ferla, Marc Garcia-Borràs, Sílvia Osuna*, Manfred T. Reetz*. Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics. Nature communications, 2021, 12, 1621.
17. Ge Qu, # Aitao Li,# Carlos G Acevedo‐Rocha,# Zhoutong Sun*, Manfred T Reetz*. The crucial role of methodology development in directed evolution of selective enzymes. Angew. Chem. Int. Ed. 2020, 59, 13204–13231.
六、专利
1. 李爱涛; 周航宇; 王婓; 余小娟; 一种P450BM3突变体及其在以苯或苯酚为底物合成对苯二酚中的应用, 2018-09-27, 中国, 201811127439.5. (专利)
2. 李爱涛; 赵晶; 王斐; 李倩; 余小娟; 彭雪盈; 陈小漫; 一种生产α,ω-二元羧酸的整细胞生物催化方法及其应用, 2020-3-3, 中国, 202010139295.6. (专利)
3. 李爱涛; 张中伟; 赵晶; 李倩; 李仁结; 王斐; 余小娟; 彭彤; α,ω-二元醇的生物催化合成方法, 2020-3-31, 中国, 202010244255.8. (专利)
4. 李爱涛，彭雅琴，高成华，赵晶，张铮斌，李纯，一种细胞色素P450BM3突变体及其在醋酸群勃龙合成中的应用，2021.08.24, 202110721653.9
5. 李爱涛，张小栋，李倩，赵晶，邓迪，一种细胞色素P450单加氧酶CYP109B2突变体及其应用，2021.05.29, 202110594533.7.
6. Aitao Li, Jing Zhao, Fei Wang, Qian Li, Xiaojuan Yu, Xueying Peng, Xiaoman Chen, Whole-cell biocatalysis method for producing α,ω-dicarboxylic acids and use thereof. 2021.01.26, US2021/0324426A1.
七、 获奖及荣誉
1. 2022，国家级青年人才
2. 2021，中国酶工程“优秀青年酶工程学家”
3. 2021，国家“十三五”科技创新成就展
4. 2021，全国商业科技进步特等奖
5. 2021，湖北大学青年科学家十大科技成果
6. 2019，湖北省杰青
7. 2018，湖北省省级人才
8. 2017，湖北省省级人才
9. 多次获优秀班主任、优秀教师奖以及连续4年获年度考核优秀
10. 导研究生获国家励志奖学金（2019）
11. 研究生获得国家留学基金委（CSC）资助，格罗宁根大学攻读博士学位
12. 指导学生获全国大学生生命科学创新创业大赛奖多次（2019-2021）
