姓名：郭瑞庭
研究方向：酶工程、结构生物学与酶学
电子邮件：guoreyting@hubu.edu.cn
通讯地址：湖北省武汉市武昌区友谊大道368号湖北大学生命科学学院
任职情况：二级教授、博士研究生导师
一、研究领域：酶分子结构、机理研究、应用与改造
郭瑞庭教授通过结构生物学方法解析重要工业酶结构，阐释催化机理，进行酶分子理性设计，以改造酶的活性与特性。工作内容主要涵盖五个体系：(1) PET塑料降解酶； (2) 新型萜类合成酶；(3) 霉菌毒素分解酶； (4) 生物质降解酶；(5) P450 酶。目前已在Nature Reviews Chemistry, Nature Catalysis, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis, PNAS 等SCI期刊发表论文130篇，共28篇获选为封面文章，申请国内外专利34个，已授权23个。2019年获天津市自然科学二等奖(第一完成人)。
二、学习和工作简历:
2018年-至今 湖北大学生命科学院、省部共建生物催化与酶工程国家重点实验室，二级教授/博导
2014-2017年 台湾政治大学EMBA，获商学硕士
2010-2018年 中科院天津工业生物技术研究所，百人计划/研究员/博导
2007-2017年 台湾基酵生物科技公司，任职研发总监/研发总顾问
2007-2009年 美国 The Scripps Research Institute 从事博士后研究
2003-2007年 台湾大学生化科学所暨台湾中研院生物化学所获博士学位
三、获奖与荣誉称号
2020年 “湖北省创新群体”(群体负责人)
2019年 “湖北省百人计划特聘专家”
2019年 “湖北省楚天学者特聘教授”
2019年 “天津市自然科学二等奖”(第一完成人)
2018年 “万人计划科技创新领军人才”
2017年 “科技部中青年科技创新领军人才”
2015年 “天津市131第一层次人才培养工程”
2012年 “天津市131第二层次人才培养工程”
2012年 “2011年科学中国人年度人物”
2010年 “中国科学院百人计划”，择优支持
2010年 为天津市特聘教授
四、 承担科研项目情况
1、 国家重大专项、2019/12-2024/11，552万、在研、主持
2、 湖北省创新群体、2020/3-2023/3，50万、在研、主持
3、 自然科学基金面上项目 (两次)、2015/01-2018/12、2019/01-2022/12、85万、在研、主持
4、 863计划、2012/8-2015/12、项目9450万、已结题、项目首席科学家
5、 973计划、2011/01-2015/08、75万、已结题、主持
6、 中科院创新交叉团队、2017/1-2019/12、100万、已结题、团队负责人
7、 中科院重点部署项目、 2013/01-2015/12、235万、已结题、主持
8、 中科院百人计划、2010年-2013年、260万、已结题、主持
9、 天津市科技项目、2011年-2014年、150万、已结题、主持
10、 企业合作开发项目 (东莞泛亚太)、2012年-2015年、255万、已结题、主持
五、代表性论文
1、 Zhang, X.,# Shen, P.,# Zhao, J.,# Chen, Y., Li, X., Huang, J.-W., Zhang, L., Li, Q., Gao, C., Xing, Q., Chen, C.-C., Guo, R.-T.,* Li, A.*. Rationally controlling selective steroid hydroxylation via scaffold sampling of a P450 Family. ACS Catalysis, 2023, 13, 1280–1289.
2、 Chen, Q.,# Li, B.,# Zhang, L.,# Chen, X., Zhu, X., Chen, F., Shi, M., Chen, C.-C., Yang, Y., Guo, R.-T.,* Liu, W., Xu, J., Zheng, G.*. Engineered imine reductase for larotrectinib intermediate manufacture. ACS Catalysis, 2022, 12 (23), 14795-14803.
3、 Dai, L.,# Li, H.,# Huang, J.-W.,# Hu, Y.,# He, M., Yang, Y., Min, J., Guo, R.-T.,* Chen, C.-C.*. Structure-based rational design of a short-chain dehydrogenase/reductase for improving activity toward mycotoxin patulin. International Journal of Biological Macromolecules, 2022-12-01, 222: 421-428.
4、 Wu, L.,# An, J.,# Jing, X.,# Chen, C.-C., Dai, L., Liu, W.,* Guo, R.-T.,* Nie, Y.*. Molecular insights into the regioselectivity of the Fe(II)/2-ketoglutarate-dependent dioxygenase-catalyzed C-H hydroxylation of amino acids. ACS Catalysis, 2022-09-08, 12: 11586-11596.
5、 Zhang, L.,# Zhang, X.,# Min, J.,# Liu, B., Huang, J.-W., Yang, Y., Liu, W., Dai, L., Yang, Y., Chen, C.-C.,* Guo, R.-T.*.. Structural insights to a bi-functional isoprenyl dihposphate synthase that can catalyze head-to-tail and head-to-middle condensation. International Journal of Biological Macromolecules, 2022-08-01, 214: 492-499.
6、 Li, H.,# Yang, Y.,# Hu, Y.,# Chen, C.-C., Huang, J.-W., Min, J., Dai, L.,* Guo, R.-T.*. Structural analysis and engineering of aldo-keto reductase from glyphosate-resistant Echinochloa colona. Journal of Hazardous Materials, 2022-08-15, 436: 129191.
7、 Malwal, S. R.,# Shang, N.,# Liu, W., Li, X., Zhang, L., Chen, C.-C., Guo, R.-T.,* Oldfield, E.*. A structural and bioinformatics investigation of a fungal squanlene synthase and comparisons with other membrane proteins. ACS Omega, 2022-06-17, 7 (26): 22601-22612.
8、 Wang, T.,# Yang, Y.,# He, M., Liu, M., Huang, J.-W., Min, J., Chen, C.-C., Liu, Y.,* Zhang, L.,* Guo, R.-T.*. Structural insights into the cyclization of unusual brasilane-type sesquiterpenes. International Journal of Biological Macromolecules, 2022-04-30, 209: 1784-1791.
9、 Dai, L.,# Zhang, X.,# Hu, Y.,# Shen, J., Zhang, Q., Zhang, L., Min, J., Chen, C.-C., Liu, Y.,* Huang, J.-W.,* Guo, R.-T.*. Structural and functional insights into a nonheme iron- and α-ketoglutarate-dependent halogenase that catalyzes chlorination of nucleotide substrates. Applied and Environmental Microbiology, 2022-04-18, 88 (9): e0249721.
10、 Zeng, W.,# Li, X.,# Yang, Y.,# Min, J.,# Huang, J.-W., Liu, W., Niu, D., Yang, X., Han, X., Zhang, L., Dai, L., Chen, C.-C.,* Guo, R.-T.*. Substrate-binding mode of a thermophilic PET hydrolase and engineering the enzyme to enhance the hydrolytic efficacy, ACS Catalysis, 2022-02-18, 12: 3033-3040.
11、 Chen, C.-C.,# Dai, M.,# Zhang, L.,# Zhao, J., Zeng, W., Shi, M., Huang, J.-W., Liu, W., Guo, R.-T.,* Li, A.*. Molecular basis for a toluene monooxygenase to govern substrate selectivity, ACS Catalysis, 2022-02-13, 12: 2831-2839.
12、 Mao, Z.,# Xiao, H.,# Shen, P.,# Yang,Y.,# Xue, J., Yang, Y., Shang, Y., Zhang, L., Li, X., Zhang, Y., Du, Y., Chen, C.-C., Guo, R.-T.,* Zhang, Y.*. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge.Cell Discovery, 2022-01-25, 8 (1): 5.
13、 Hu, Y.#, Li, H.,# Min, J.,# Yu, Y., Liu, W., Huang, J.-W., Zhang, L., Yang, Y., Dai, L.*, Chen, C.-C.,* Guo, R.-T.*. Crystal structure and biochemical analysis of the specialized deoxynivalenol–detoxifying glyoxalase SPG from Gossypium hirsutum. International Journal of Biological Macromolecules, 2022-01-18, 200: 388-396.
14、 陈纯琪, 韩旭, 刘卫东, 马立新, 刘珂*，郭瑞庭*. 基于结构改造来源于大阪伊德氏杆菌 201-F6 的PET水解酶，生物工程学报，2021-09-25, 37 (9): 3268-3275.
15、 Dai, L.,# Chang, Z.,# Yang, J., Liu,W., Yang, Y., Chen, C.-C., Zhang, L., Huang, J.-W.,* Sun, Y.,* Guo, R.-T.*. Structural investigation of a thermostable 1,2- β -mannobiose phosphorylase from Thermoanaerobacter sp. X-514. Biochemical and Biophysical Research Communications, 2021-09-21, 579: 54-61.
16、 Dai, L.,# Qu, Y.,# Hu, Y.,# Min, J., Yu, X., Chen, C.-C., Huang, J.-W.,* Guo, R.-T.*. Catalytically inactive lytic polysaccharide monooxygenase PcAA14A enhances the enzyme-mediated hydrolysis of polyethylene terephthalate, International Journal of Biological Macromolecules, 2021-09-07, 190: 456-462.
17、 Dai, L.,# Qu, Y.,# Huang, J.-W., Hu, Y., Hu, H., Li, S., Chen, C.-C.,* Guo, R.-T.*.Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021-05-24, 334: 47-50.
18、 Liu, W.,# Ma, C.,# Liu, W.,# Zheng, Y., Chen, C.-C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R.-T.,* Zhang, T.*. Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5. International Journal of Biological Macromolecules, 2021-04-15, 182: 899-909.
19、Chen, C. C., Han, X., Li, X., Jiang, P., Niu, D., Ma, L., Liu, W., Li, Siyu, Qu, Y., Hu, H., Min, J., Yang, Y., Zhang, L., Zeng, W., Huang, J. W.,* Dai, L.,* Guo, R. T.* General features to enhance enzymatic activity of poly (ethylene terephthalate) hydrolysis, Nature Catalysis, 2021, 4, 425-430 (Highlighted by ChemistryViews, 科技日报, 中国科学报)
20、 Chen, C. C., Yu, X., Kuo, C.J., Min, J., Chen, S., Ma, L.,* Liu, K.,* Guo, R. T.* Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal, 2021, in press. (Review, https://doi.org/10.1111/febs.15696)
21、 Dai, L., Qu, Y., Huang, J. W., Hu, Y., Hu, H., Li, S., Chen, C. C.,* Guo, R. T.* Enhancing PET hydrolytic enzyme activity by fusion of the cellulose-binding domain of cellobiohydrolase I from Trichoderma reesei. Journal of Biotechnology, 2021, 334, 47-50.
22、 Liu, W., Ma, C., Liu, W., Zheng, Y., Chen, C. C., Liang, A., Luo, X., Li, Z., Ma, W., Song, Y.,* Guo, R. T.,* Zhang, T.* Functional and structural investigation of a novel β-mannanase BaMan113A from Bacillus sp. N16-5. International Journal of Biological Macromolecules, 2021, 182, 899-909
23、 Chen, C. C., Min, J., Zhang, L., Yang, Y., Yu, X.,* Guo, R. T.* Advanced Understanding of the Electron Transfer Pathway of Cytochrome P450s. ChemBioChem, 2021, 22, 1317-1328 (Review, Cover story)
24、Chen, C. C., Malwal, S. R., Han, X., Liu, W., Ma, L., Zhai, C., Dai, L., Huang, J. W., Shillo, A., Desai, J., Ma, X., Zhang, Y., Guo, R. T.,* Oldfield, E.* Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action. ACS Catalysis, 2021, 11, 290-303 (Cover story)
25、Zhang, L., Xie, Z., Liu, Z., Zhou, S., Ma, L., Liu, W., Huang, J. W., Ko, T. P., Li, X., Hu, Y., Min, J., Yu, X., Guo, R. T.,* Chen, C. C.* Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications, 2020, 11, 2676. (Highlighted by C&EN, ChemistryViews, 科技日报, 中国科学报)
26、 Chen, C. C.,* Zhang, L., Yu, X., Ma, L., Ko, T. P., Guo, R. T.* Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon–carbon bond formation. ACS Catalysis, 2020, 10, 4717-4725. (Review, ACS Editors’ Choice, Cover story)
27、 Chen, C. C., Dai, L., Ma, L.,* Guo, R. T.* Enzymatic degradation of plant biomass and synthetic polymers. Nature Reviews Chemistry, 2020, 4, 114-126. (Review, Cover story)
28、 Song, M., Zhang, X., Liu, W.,* Feng, J.,* Cui, Y., Yao, P., Wang, M., Guo, R. T., Wu, Q., Zhu, D.* 2,3-Dihydroxybenzoic Acid Decarboxylase from Fusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism. ChemBioChem, 2021, 21, 2950-2956.
29、 Huang, J. W., Niu, D., Liu, K., Wang, Q., Ma, L., Chen, C. C., Zhang, L., Liu, W., Zhou, S., Min, J., Wu, S., Yang, Y.,* Guo, R. T.* Structure basis of non-structural protein pA151R from African Swine Fever Virus. Biochemical and Biophysical Research Communications, 2020, 532, 108-113.
30、 Liu, Y., Lee, C., Li, F., Trček, J., Bähre, H., Guo, R. T., Chen, C. C., Chernobrovkin, A., Zubarev, R., Römling, U. A Cyclic di-GMP Network Is Present in Gram-Positive Streptococcus and Gram-Negative Proteus Species. ACS Infectious Diseases, 2020, 6, 2672-2687.
31、 Xiao, X., Elsayed, S. S., Wu, C., van der Heul, H. U., Metsä-Ketelä, M., Du, C., Prota, A. E., Chen, C. C., Liu, W., Guo, R. T., Abrahams, J. P., van Wezel, G. P. Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway. ACS Chemical Biology, 2020, 15, 2529-2538
32、 Zhou, S., Ko, T. P.,* Huang, J. W., Liu, W., Zheng, Y., Wu, S., Wang, Q., Xie, Z., Liu, Z., Chen, C. C.,* Guo, R. T.* Structure of a gut microbial Diltiazem-metabolizing enzyme suggests possible substrate binding mode. Biochemical and Biophysical Research Communications, 2020, 527, 799-804.
33、 Chen, X., Zhang, H., Maria-S., Miguel A., Liu, W., Li, J., Feng, J., Liu, X., Osuna S.,* Guo, R. T., Wu, Q.,* Zhu, D.,* Ma, Y. Efficient reductive desymmetrization of bulky 1, 3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase, Nature Catalysis, 2019, 2, 931-941.
34、 Yang, Y., Li, L., Yuan, L., Zhou, X., Duan, J., Xiao, H., Cai, N., Han, S., Ma, X., Liu, W., Chen, C. C., Wang, L., Li, X., Chen, J., Kang, N., Chen, J., Shen, Z., Malwal, S. R., Liu, W., Shi, Y., Oldfield, E., Guo, R. T.,* Zhang, Y.* A structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity, 2019, 50, 1043-1053
35、 Sun, H., Ko, T. P., Liu, W., Liu, W., Zheng, Y., Chen, C. C.,* Guo, R. T.* Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications, 2019, 521, 31-36.
36、 Kuo, C. J., Gao, J., Huang, J. W., Ko, T. P., Zhai, C., Ma, L., Liu, W., Dai, L., Chang, Y. F., Chen, T. H., Hu, Y., Yu, X., Guo, R. T.,* Chen, C. C.* Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. Biochimica et Biophysica Acta. General subjects, 2019, 1863, 1351-1359
37、 Tang, X.# Xue, J.#, Yang, Y., Ko, T. P., Chen, C.Y., Dai, L., Guo, R.T. , Zhang, Y.,* Chen C. C.* Structural insights into the calcium dependence of Stig cyclases. RSC Advances, 2019, 9, 13182.
38、 Ma, J., Ko, T. P., Yu, X.,Zhang, L., Ma, L., Zhai, C., Guo, R. T., Liu, W., Li, H., Chen, C. C.* Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications, 2019, 515, 621-626.
39、 Zhang, L., Ko, T. P., Malwal S. R., Liu W., Zhou S., Oldfield E., Guo R. T.,* and Chen, C. C.* Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism, Biochemical and Biophysical Research Communications, 2019, 511, 800-805
40、 Chang, Z., Ansbacher, T., Zhang, L., Yang, Y., Ko, T. P., Zhang, G., Liu, W., Huang, J. W., Dai, L., Guo, R. T., Major, D. T.,* Chen, C. C.* Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis, Organic Biomolecular Chemistry, 2019, 17, 2070-2076. (Cover story)
41、 Malwal, S. R., Chen, L., Hicks, H., Qu, F., Liu, W., Shillo, A., Law, W.X., Zhang, J., Chandnani, N., Han, X., Zheng, Y., Chen, C. C., Guo, R. T., AbdelKhalek, A., Seleem, M. N., Oldfield, E. Discovery of lipophilic bisphosphonates that target bacterial cell wall and quinone biosynthesis. Journal of Medicinal Chemistry, 2019, 62, 2564-2581.
42、 Zhang, M., Yu, X. W., Xu, Y., Guo, R. T., Swapna, GVT, Szyperski, T., Hunt, J. F., Montelione, G. T. Structural Basis by Which the N-Terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity. Biochemistry, 2019, 58, 3943-3954.
43、 Chen, C. C.,# Hu, X.,# Tang, X.,# Yang, Y.,# Ko, T. P., Gao, J., Zheng, Y., Huang, J. W., Yu, Z., Li, L., Han, S., Cai, N., Zhang, Y.,* Liu, W.,* Guo, R. T.* The crystal structure of a class of cyclases that catalyze the Cope rearrangement. Angewandte Chemie International Edition, 2018, 57, 15060-15064. (Cover story)
44、Gao, J.,# Ko, T. P.,# Chen, L.,# Malwal, S. R., Zhang, J., Hu, X., Qu, F., Liu, W., Huang, J. W., Cheng, Y. S., Chen, C. C., Yang, Y., Zhang, Y., Oldfield, E., Guo, R. T.* “Head-to-middle” and “head-to-tail” cis-prenyl transferases: Structure of isosesquilavandulyl diphosphate synthase. Angewandte Chemie International Edition, 2018, 57, 683-687. (Cover story)
45、 Chen, C. C.,# Han, X.,# Ko, T. P., Liu, W.,* Guo, R. T.* Structural studies reveal the molecular mechanism of PETase. FEBS Journal, 2018, 285, 3717-3723.
46、 You, S., Chen, C. C., Tu, T., Wang, X., Ma, R., Cai, H. Y., Guo, R. T.,* Luo, H. Y.,* Yao, B.* Insight into the functional roles of Glu175 in the hyperthermostable xylanase XYL10C-ΔN through structural analysis and site-saturation mutagenesis. Biotechnol Biofuels, 2018, 11, 159.
47、 Zheng, Y.,# Liu, W.,# Chen C. C.,# Hu, X., Liu, W., Ko, T. P., Tang, X., Wei, H., Huang, J. W., Guo, R. T.* Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei: Molecular insight into ZHD substrate selectivity. ACS Catalysis. 2018, 8, 4294-4298.
48、 Malwal, S. R.,# Gao, J.,# Hu, X.,# Yang, Y.,# Liu, W., Huang J. W., Ko, T. P., Li, L., Chen, C. C., O’Dowd, B., Khade, R. L., Zhang, Y., Zhang, Y., Oldfield, E., Guo, R. T.* Catalytic role of conserved asparagine, glutamine, serine, and tyrosine residues in isoprenoid biosynthesis enzymes ACS Catalysis. 2018, 8, 4299-4312.
49、 Lee, Suki Man-Yan, Yip, T., Yan, S., Jin, D., Wei, H., Guo, R. T., Peiris, JS Malik,* Recognition of double-stranded RNAand regulation of interferon pathway by Toll-likereceptor10. Frontiers in Immunology. 2018, 9, 516.
50、Wang, J.,# Chen, C. C.,# Yang, Y.,# Liu, W., Ko, T. P., Shang, N., Hu, X., Xie, Y., Huang, J. W., Zhang, Y.,* Guo, R. T.* Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis. Biochemical and Biophysical Research Communications, 2018, 495, 1782-1788.
51、Han, X.,# Liu, W.,# Huang, J. W.,# Ma, J., Zheng, Y., Ko, T. P., Xu, L., Cheng, Y. S., Chen, C. C., Guo, R. T.* Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 2017, 13, 2106.
52、 Gao, J.,# Huang, J. W.,# Li, Q.,# Liu, W., Ko, T. P., Zheng, Y., Xiao, X., Kuo, C. J., Chen, C. C.,* Guo, R. T.* Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-β-endoglucanase from Thielavia terrestris. Enzyme and Microbial Technology, 2017, 99, 32-37.
53、 Schwalen, C. J., Feng, X., Liu, W., O-Dowd, B., Ko, T. P., Shin, C. J., Guo, R. T., Mitchell, D. A.,* Oldfield, E.* Head-to-head prenyl synthases in pathogenic bacteria. ChemBioChem : a European Journal of Chemical Biology, 2017, 18, 985-991.
54、 Hui, R.,# Hu, X.,# Liu, W., Zheng, Y., Chen, Y., Guo, R. T., Jin, J.,* Chen, C. C.* Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana. Acta Crystallographica. Section F, Structural Biology Communications, 2017, 73, 515-519.
55、 Zhang, L.,# Chen, C. C.,# Ko, T. P.,# Huang, J. W., Zheng, Y., Liu, W., Wang, I., Malwal, S. R., Feng, X., Wang, Ke., Huang, C. C., Hsu, S. T., Wang, A. H., Oldfield, E.,* and Guo, R. T.* Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5, Angewandte Chemie International Edition, 2016, 55, 4716-4720.
56、 Xu, Z.,# Liu, W.,# Chen, C. C.,# Li Q., Huang, J. W., Ko, T. P., Liu, G., Liu, W., Peng, W., Cheng, Y. S., Chen, Y., Jin, J., Li, H.,* Zheng, Y.,* Guo, R. T.* Enhanced α‑zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering. ACS Catalysis, 2016, 6, 7657-7663. (Cover story)
57、 Liu, M.,# Chen, C. C.,# Chen, Lu.,# Xiao, X., Zheng, Y.,Huang, J. W., Liu, W., Ko, T. P., Cheng, Y. S., Feng, X., Oldfield, E.,* Guo, R. T.,* and Ma, Y.* Structure and function of a “head-to-middle” prenyltransferase: Lavandulyl diphosphate Synthase, Angewandte Chemie International Edition, 2016, 55, 4721-4724. (Cover story)
58、 Wang, Y., Desai, J., Zhang, Y., Malwal, S. R., Shin, C. J., Feng, X., Sun, H., Liu, G., Guo, R. T., Oldfield, E.* Bacterial dell growth inhibitors targeting undecaprenyl diphosphate synthase and undecaprenyl diphosphate phosphatase. ChemMedChem, 2016, 11, 2311-2319.
59、 Yan, J.,# Liu, W.,# Li, Y., Lai, H. L., Zheng, Y., Huang, J. W., Chen, C. C., Chen, Y., Jin, J., Li, H.,* Guo, R. T.* Functional and structural analysis of Pichia pastoris expressed Aspergillus niger 1,4-β-endoglucanase. Biochemical and Biophysical Research Communications, 2016, 17, 8-12.
60、 Desai, J., Liu, Y. L., Wei, H., Liu, W., Ko, T. P., Guo, R. T., Oldfield, E.* Structure, function, and inhibition of Staphylococcus aureus heptaprenyl diphosphate synthase. ChemMedChem, 2016, 11, 1915-1923.
61、 Huang, J. W.,# Liu, W.,# Lai, H. L., Cheng, Y. S., Zheng, Y., Li, Q., Sun, H., Kuo, C. J., Guo, R. T.,* Chen, C. C.* Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications, 2016, 16, 565-572.
62、 Yang, Y.,# Ko, T. P.,# Chen, C. C.,# Huang, G., Zheng, Y., Liu, W., Wang, I., Ho, M., Hsu, S., O'Dowd, B., Huff, H., Huang, C. H., Docampo, R., Oldfield, E.,* Guo, R. T.,* Structures of trypanosome vacuolar soluble pyrophosphatases: Anti-parasitic drug targets. ACS Chemical Biology, 2016, 11, 1362-1371.
63、 Zheng, Y.,# Li, Y.,# Liu, W.#, Chen, C. C., Ko, T. P., He, M., Xu, Z., Liu, M., Luo, H., Guo, R. T.,* Yao B,* and Ma Y.* Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-β-1, 4-xylanase. Journal of Structural Biology, 2016, 193, 206-211. (Cover story)
64、 Liu, G., Li, Q., Shang, N., Huang J. W., Ko, T. P., Liu, W., Zheng, Y., Han, X., Chen, Y., Chen, C. C., Jin, J., Guo, R. T.* Functional and structural analyses of a 1,4- β-endoglucanase from Ganoderma lucidum. Enzyme and Microbial Technology, 2016, 86, 67-74.
65、 Hu, Y.,# Liu, W.,# Malwal, S. R.,Zheng, Y., Feng, X., Ko, T. P., Chen, C. C., Xu, Z., Liu, M., Han, X., Gao, J., Oldfield, E.,* Guo, R. T.* Structures of iridoid synthase from Cantharanthus roseus with bound NAD(+), NADPH, or NAD(+) /10-oxogeranial: Reaction mechanisms. Angewandte Chemie International Edition, 2015, 54, 15478-15482. (Cover story)
66、 Cheng, Y. S., Chen, C. C., Huang, J. W., Ko, T. P., Huang, Z., Guo, R. T.* Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Applied Microbiology and Biotechnology, 2015, 99, 9503-9510.
67、 Zheng, Y., Chen, C. C., Ko, T. P., Xiao, X., Yang, Y., Huang, C. H., Qian, G., Shao, W.,* Guo, R. T.* Crystal structures of S-adenosyl homocysteine hydrolase from the thermophilic bacterium Thermotoga maritima. Journal of Structural Biology, 2015, 190, 135-142. (Cover story)
68、 Guo, R. T.* Green Enzymes. ChemBioEng Reviews, 2015, 2, 75. (Invited Editorial)
69、 Chen, C. C., Cheng, K. J., Ko, T. P., Guo, R. T.* Current progresses in phytase research: Three-dimensional structure and protein engineering. ChemBioEng Reviews, 2015, 2, 76-86. (Cover story)
70、 Chen, C. C., Ko, T. P., Huang, J. W., Guo, R. T.* Heat- and alkaline-stable xylanases: applications, protein structure and engineering. ChemBioEng Reviews, 2015, 2, 95-106.
71、 Chan, H. C.,# Ko, T. P.,# Huang, C. H., Guo, R. T.* Minireview: A comeback of Hg-derivatives in protein crystallography with Cys-modification. ChemBioEng Reviews, 2015, 2, 133-140.
72、 Liu, W., Guo, R. T., Chen, X., Li, Z., Gao, X., Huang, C. H., Wu, Q., Feng, J., Zhu, D.* Structural analysis reveals the substrate-binding mechanism for the expanded substrate specificity of mutant meso-diaminopimelate dehydrogenase. ChemBioChem : a European Journal of Chemical Biology, 2015, 16, 924-929. (Cover story)
73、 Chen, Y., Huang, J. W., Chen, C. C., Lai, H. L., Jin, J., Guo, R. T.* Crystallization and preliminary X-ray diffraction analysis of an endo-1, 4-beta-D-glucanase from Aspergillus aculeatus F-50. Acta Crystallographica. Section F, Structural Biology Communications, 2015, 71, 397-400.
74、 Zhu, W., Wang, Y., Li, K., Gao, J., Huang, C. H., Chen, C. C., Ko, T. P., Zhang, Y., Guo, R. T., Oldfield, E.* Antibacterial drug leads: DNA and enzyme multi-targeting. Journal of Medicinal Chemistry, 2015, 58, 1215-1227.
75、 Zheng, Y., # Ko, T. P., # Yang, Y., Shao, W., * Guo, R. T.,* Binding mode of the oxidized alpha-anomer of NAD+ to RSP, a Rex-family repressor. Biochemical and Biophysical Research Communications, 2015, 456, 733-736.
76、 Yuan, S. F., Wu, T. H., Lee, H. L., Hsieh, H. Y., Lin, W. L., Yang, B., Chang, C. K., Li, Q., Gao, J., Huang, C. H., Ho, M. C.,* Guo, R. T.,* Liang, P. H.* Biochemical characterization and structural analysis of a bi-functional cellulase/xylanase from Clostridium thermocellum. The Journal of Biological Chemistry, 2015, 290, 5739-5748.
77、 Chen, C. C., Huang, J. W., Zhao, P., Ko, T. P., Huang, C. H., Chan, H. C., Huang, Z., Liu, W., Cheng, Y. S., Liu, J. R., Guo, R. T.* Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum. Enzyme and Microbial Technology, 2015, 71, 1-7.
78、 Veiga-Santos, P., Li, K., Lameira, L., de Carvalho, T. M., Huang, G., Galizzi, M., Shang, N., Li, Q., Gonzalez-Pacanowska, D., Hernandez-Rodriguez, V., Benaim, G., Guo, R. T., Urbina, J. A., Docampo, R., de Souza, W., Oldfield, E.* SQ109: A new drug lead for Chagas disease. Antimicrobial Agents and Chemotherapy, 2015, 59, 1950-1961.
79、 Han, X., Chen, C. C., Kuo, C. J., Huang, C. H., Zheng, Y., Ko, T. P., Zhu, Z., Feng, X., Wang, K., Oldfield, E., Wang, A. H., Liang, P. H., Guo, R. T.,* Ma, Y.* Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins, 2015, 83, 37-45.
80、 Chan, H. C.,# Feng, X.,# Ko, T. P.#, Huang, C. H., Hu, Y., Zheng, Y., Bogue, S., Nakano, C., Hoshino, T., Zhang, L., Lv, P., Liu, W., Crick, D. C., Liang, P. H., Wang, A. H., Oldfield, E.,* Guo, R. T.* Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis. Journal of the American Chemical Society, 2014, 136, 2892-2896.
81、 Liu, W., #Feng, X., # Zheng, Y., # Huang, C. H., Nakano, C., Hoshino, T., Bogue, S., Ko, T. P., Chen, C. C., Cui, Y., Li, J., Wang, I., Hsu, S. T., Oldfield, E.,* Guo, R. T.* Structure, function and inhibition of ent-kaurene synthase from Bradyrhizobium japonicum. Scientific Reports, 2014, 4, 6214.
82、 Cheng, Y. S., # Chen, C. C., # Huang, C. H., Ko, T. P., Luo, W., Huang, J. W., Liu, J. R., Guo, R. T.* Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme. The Journal of Biological Chemistry, 2014, 289, 11020-11028.
83、 Shang, N., # Li, Q., # Ko, T. P., # Chan, H. C., Li, J., Zheng, Y., Huang, C. H., Ren, F., Chen, C. C., Zhu, Z., Galizzi, M., Li, Z. H., Rodrigues-Poveda, C. A., Gonzalez-Pacanowska, D., Veiga-Santos, P., de Carvalho, T. M., de Souza, W., Urbina, J. A., Wang, A. H., Docampo, R., Li, K., Liu, Y. L., Oldfield, E.,* Guo, R. T.* Squalene synthase as a target for Chagas disease therapeutics. PLoS Pathogens, 2014, 10, e1004114.
84、 Zheng, Y., # Ko, T. P., # Sun, H., Huang, C. H., Pei, J., Qiu, R., Wang, A. H., Wiegel, J., Shao, W.,* Guo, R. T.* Distinct structural features of Rex-family repressors to sense redox levels in anaerobes and aerobes. Journal of Structural Biology, 2014, 188, 195-204. (Cover story)
85、 Peng, W., # Ko, T. P., # Yang, Y., Zheng, Y., Chen, C. C., Zhu, Z., Huang, C. H., Zeng, Y. F., Huang, J. W., Wang, A. H.-J., Liu, J. R.,* Guo, R. T.* Crystal structure and substrate-binding mode of the mycoestrogen-detoxifying lactonase ZHD from Clonostachys rosea. RSC Advances, 2014, 4, 62321-62325.
86、 Zhu, Z., # He, M., # Huang, C. H., Ko, T. P., Zeng, Y. F., Huang, Y. N., Jia, S., Lu, F., Liu, J. R., Guo, R. T.* Crystallization and preliminary X-ray diffraction analysis of a novel beta-L-arabinofuranosidase (HypBA1) from Bifidobacterium longum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 636-638.
87、 Huang, C. H., Zhu, Z., Cheng, Y.S., Chan, H.C., Ko, T.P., Chen, C. C., Wang, I., Ho, M. R., Hsu, S. T. -D., Zeng, Y. F., Huang, Y. N., Liu, J. R.,* Guo, R. T.* Structure and Catalytic Mechanism of a Glycoside Hydrolase Family-127β-L-Arabinofuranosidase (HypBA1). J Bioprocess Biotech, 2014, 4, 171.
88、 Zhang, L., # Zhao, P., # Chen, C. C., Huang, C. H., Ko, T. P., Zheng, Y., Guo, R. T.* Preliminary X-ray diffraction analysis of a thermophilic beta-1,3-1,4-glucanase from Clostridium thermocellum. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 946-948.
89、 Yang, Y., # Ko, T. P., # Liu, L., Li, J., Huang, C. H., Chen, J., Guo, R. T.,* Du, G.* Roles of tryptophan residue and disulfide bond in the variable lid region of oxidized polyvinyl alcohol hydrolase. Biochemical and Biophysical Research Communications, 2014, 452, 509-514.
90、 Yang, Y., # Ko, T. P., # Liu, L., Li, J., Huang, C. H., Chan, H. C., Ren, F., Jia, D., Wang, A. H., Guo, R. T.,* Chen, J.,* Du, G.* Structural insights into enzymatic degradation of oxidized polyvinyl alcohol. ChemBioChem : a European Journal of Chemical Biology, 2014, 15, 1882-1886. (Cover story)
91、 Xu, J., # Ren, F., # Huang, C. H., Zheng, Y., Zhen, J., Sun, H., Ko, T. P., He, M., Chen, C. C., Chan, H. C., Guo, R. T.,* Song, H.,* Ma, Y.* Functional and structural studies of pullulanase from Anoxybacillus sp. LM18-11. Proteins, 2014, 82, 1685-1693. (Cover story)
92、 Wu, T. H., # Chen, C. C., # Cheng, Y. S., Ko, T. P., Lin, C. Y., Lai, H. L., Huang, T. Y., Liu, J. R., * Guo, R. T.* Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. Journal of Biotechnology, 2014, 175, 1-6.
93、 Wang, S., Nie, Y., Xu, Y.,* Ko, T. P., Huang, C. H., Chan, H. C., Guo, R. T.,* Xiao, R. Crystallization and preliminary X-ray diffraction analysis of (R)-carbonyl reductase from Candida parapsilosis. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 800-802.
94、 Wang, S., Nie, Y., Xu, Y.,* Zhang, R., Ko, T. P., Huang, C. H., Chan, H. C., Guo, R. T.,* Xiao, R. Unconserved substrate-binding sites direct the stereoselectivity of medium-chain alcohol dehydrogenase. Chemical Communications, 2014, 50, 7770-7772. (Cover story)
95、 Tseng, C. W., # Liao, C. Y., # Sun, Y., Peng, C. C., Tzen, J. T., Guo, R. T.,* Liu, J. R.* Immobilization of Clostridium cellulolyticum D-psicose 3-epimerase on artificial oil bodies. Journal of Agricultural and Food Chemistry, 2014, 62, 6771-6776.
96、 Lv, P., # Zhang, L., # Luo, H., Chen, C. C., Huang, C. H., Peng, W., Wang, K., Ko, T. P., Zheng, Y., Zhang, J., Yao, B.,* Guo, R. T.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-xylanase from Streptomyces sp. S9. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 105-107.
97、 Liu, W., Li, Z., Huang, C. H., Guo, R. T., Zhao, L., Zhang, D., Chen, X., Wu, Q., Zhu, D.* Structural and mutational studies on the unusual substrate specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum. ChemBioChem : a European Journal of Chemical Biology, 2014, 15, 217-222. (Cover story)
98、 Li, K., Schurig-Briccio, L. A., Feng, X., Upadhyay, A., Pujari, V., Lechartier, B., Fontes, F. L., Yang, H., Rao, G., Zhu, W., Gulati, A., No, J. H., Cintra, G., Bogue, S., Liu, Y. L., Molohon, K., Orlean, P., Mitchell, D. A., Freitas-Junior, L., Ren, F., Sun, H., Jiang, T., Li, Y., Guo, R. T., Cole, S. T., Gennis, R. B., Crick, D. C., Oldfield, E.* Multitarget drug discovery for tuberculosis and other infectious diseases. Journal of Medicinal Chemistry, 2014, 57, 3126-3139.
99、 Huang, J. W., # Chen, C. C., # Huang, C. H., Huang, T. Y., Wu, T. H., Cheng, Y. S., Ko, T. P., Lin, C. Y., Liu, J. R.,* Guo, R. T.,* Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design. Biochimica et Biophysica Acta, 2014, 1844, 663-669. (Cover story)
100、 He, M., # Zheng, Y., # Huang, C. H., Qian, G., Xiao, X., Ko, T. P., Shao, W., Guo, R. T.* Crystallization and preliminary X-ray diffraction analysis of the S-adenosylhomocysteine hydrolase (SAHH) from Thermotoga maritima. Acta Crystallographica. Section F, Structural Biology Communications, 2014, 70, 1563-1565.
101、 Feng, X., # Hu, Y., # Zheng, Y., # Zhu, W., Li, K., Huang, C. H., Ko, T. P., Ren, F., Chan, H. C., Nega, M., Bogue, S., Lopez, D., Kolter, R., Gotz, F., Guo, R. T.,* Oldfield, E.* Structural and functional analysis of Bacillus subtilis YisP reveals a role of its product in biofilm production. Chemistry & Biology, 2014, 21, 1557-1563.
102、 Cheng, Y. S., # Huang, C. H., # Chen, C. C., Huang, T. Y., Ko, T. P., Huang, J. W., Wu, T. H., Liu, J. R.,* Guo, R. T.,* Structural and mutagenetic analyses of a 1,3-1,4-beta-glucanase from Paecilomyces thermophila. Biochimica et Biophysica Acta, 2014, 1844, 366-373. (Cover story)
103、 Chen, C. C., # Luo, H., # Han, X., # Lv, P., Ko, T. P., Peng, W., Huang, C. H., Wang, K., Gao, J., Zheng, Y., Yang, Y., Zhang, J., Yao, B.,* Guo, R. T.* Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Journal of Biotechnology, 2014, 189, 175-182.
104、 Bai, W., Zhou, C., Xue, Y., Huang, C. H., Guo, R. T., Ma, Y.* Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its thermal performance by introducing arginines substitutions. Biotechnology Letters, 2014, 36, 1495-1501.
105、 Ren, F., # Feng, X., # Ko, T. P., # Huang, C. H., Hu, Y., Chan, H. C., Liu, Y. L., Wang, K., Chen, C. C., Pang, X., He, M., Li, Y., Oldfield, E.,* Guo, R. T.* Insights into TIM-barrel prenyl transferase mechanisms: crystal structures of PcrB from Bacillus subtilis and Staphylococcus aureus. ChemBioChem : a European Journal of Chemical Biology, 2013, 14, 195-199. (Cover story)
106、 Luo, W., # Huang, J. W., # Huang, C. H., Huang, T. Y., Chan, H. C., Liu, J. R., Guo, R. T., Chen, C. C.* Preliminary X-ray diffraction analysis of thermostable beta-1,4-mannanase from Aspergillus niger BK01. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 1100-1102.
107、 Li, X., # Han, X., # Ko, T. P., Chen, C. C., Zhu, Z., Hua, E., Guo, R. T., Huang, C. H. * Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase from Escherichia coli. Acta crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 328-331.
108、 Jiang, T., # Chan, H. C., # Huang, C. H., Ko, T. P., Huang, T. Y., Liu, J. R., Guo, R. T.* Substrate binding to a GH131 beta-glucanase catalytic domain from Podospora anserina. Biochemical and Biophysical Research Communications, 2013, 438, 193-197.
109、 Hu, Y., Jia, S., Ren, F., Huang, C. H., Ko, T. P., Mitchell, D. A., Guo, R. T., Zheng, Y.* Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp. subtilis strain 168. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2013, 69, 77-79.
110、 Han, X., # Gao, J., # Shang, N., # Huang, C. H., Ko, T. P., Chen, C. C., Chan, H. C., Cheng, Y. S., Zhu, Z., Wiegel, J., Luo, W.,*Guo, R. T.,* Ma, Y.* Structural and functional analyses of catalytic domain of GH10 xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Proteins, 2013, 81, 1256-1265. (Cover story)
111、 Chang, T. H., # Chang, S. J., # Hsieh, F. L., Ko, T. P., Lin, C. T., Ho, M. R., Wang, I., Hsu, S. T., Guo, R. T., Chang, W., Wang, A. H.* Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. PLoS Pathogens, 2013, 9, e1003563.
112、 Ren, F.,# Ko, T. P.,# Feng, X., Huang, C. H., Chan, H. C., Hu, Y., Wang, K., Ma, Y., Liang, P. H., Wang, A. H., Oldfield, E.,* Guo, R. T.* Insights into the mechanism of the antibiotic-synthesizing enzyme MoeO5 from crystal structures of different complexes. Angewandte Chemie International Edition, 2012, 51, 4157-4160. (Cover story)
113、 Zheng, Y., Huang, C. H., Liu, W., Ko, T. P., Xue, Y., Zhou, C., Guo, R. T., Ma, Y.* Crystal structure and substrate-binding mode of a novel pectate lyase from alkaliphilic Bacillus sp. N16-5. Biochemical and Biophysical Research Communications, 2012, 420, 269-274.
114、 Liu, W., Sun, Y., Ko, T. P., Wiegel, J., Shao, W., Lu, F., Guo, R. T., Huang, C. H.* Crystallization and preliminary X-ray diffraction analysis of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2012, 68, 914-916.
115、 Huang, J. W., Cheng, Y. S., Ko, T. P., Lin, C. Y., Lai, H. L., Chen, C. C., Ma, Y., Zheng, Y., Huang, C. H., Zou, P., Liu, J. R.,* Guo, R. T.* Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Applied Microbiology and Biotechnology, 2012, 94, 111-121.
116、 Huang, C. H.,# Sun, Y.,# Ko, T. P., Chen, C. C., Zheng, Y., Chan, H. C., Pang, X., Wiegel, J.,* Shao, W.,* Guo, R. T.* The substrate/product-binding modes of a novel GH120 beta-xylosidase (XylC) from Thermoanaerobacterium saccharolyticum JW/SL-YS485. The Biochemical Journal, 2012, 448, 401-407.
117、 Cheng, Y. S., Ko, T. P., Huang, J. W., Wu, T. H., Lin, C. Y., Luo, W., Li, Q., Ma, Y., Huang, C. H., Wang, A. H., Liu, J. R.,* Guo, R. T.* Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop. Applied Microbiology and Biotechnology, 2012, 95, 661-669.
118、 Chang, K. M., Chen, S. H., Kuo, C. J., Chang, C. K., Guo, R. T., Yang, J. M., Liang, P. H.* Roles of amino acids in the Escherichia coli octaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis. Biochemistry, 2012, 51, 3412-3419.
119、 Chan, H. C.,# Zhu, Y.,# Hu, Y., Ko, T. P., Huang, C. H., Ren, F., Chen, C. C., Ma, Y., Guo, R. T.,* Sun, Y.* Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein & Cell, 2012, 3, 123-131. (Cover story)
120、 Pai, C. K. Wang, H. T., Guo, R. T., Liu, J. R.* The construction of bifunctional fusion xylanolytic enzymes and the prediction of optimum reaction conditions for the enzyme activity. BioResources, 2012, 7, 5647-5665.
121、 Zeng, Y. F., # Ko, T. P., # Lai, H. L., Cheng, Y. S., Wu, T. H., Ma, Y., Chen, C. C., Yang, C. S., Cheng, K. J., Huang, C. H., Guo, R. T.,* Liu, J. R.* Crystal structures of Bacillus alkaline phytase in complex with divalent metal ions and inositol hexasulfate. Journal of Molecular Biology, 2011, 409, 214-224. (Cover story)
122、 Wu, T. H., # Huang, C. H., # Ko, T. P., Lai, H. L., Ma, Y., Chen, C. C., Cheng, Y. S., Liu, J. R.,* Guo, R. T.* Diverse substrate recognition mechanism revealed by Thermotoga maritima Cel5A structures in complex with cellotetraose, cellobiose and mannotriose. Biochimica et Biophysica Acta, 2011, 1814, 1832-1840. (Cover story)
123、 Tseng, C. W.,# Ko, T. P.,#Guo, R. T.,# Huang, J. W., Wang, H. C., Huang, C. H., Cheng, Y. S., Wang, A. H., Liu, J. R.* Substrate binding of a GH5 endoglucanase from the ruminal fungus Piromyces rhizinflata. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 2011, 67, 1189-1194. (Cover story)
124、 Cheng, Y. S., Ko, T. P., Wu, T. H., Ma, Y., Huang, C. H., Lai, H. L., Wang, A. H., Liu, J. R.,* Guo, R. T.* Crystal structure and substrate-binding mode of cellulase 12A from Thermotoga maritima. Proteins, 2011, 79, 1193-1204. (Cover story)
125、 Zhang, Y., Cao, R., Yin, F., Hudock, M. P., Guo, R. T., Krysiak, K., Mukherjee, S., Gao, Y. G., Robinson, H., Song, Y., No, J. H., Bergan, K., Leon, A., Cass, L., Goddard, A., Chang, T. K., Lin, F. Y., Van Beek, E., Papapoulos, S., Wang, A. H., Kubo, T., Ochi, M., Mukkamala, D., Oldfield, E.* Lipophilic bisphosphonates as dual farnesyl/geranylgeranyl diphosphate synthase inhibitors: an X-ray and NMR investigation. Journal of the American Chemical Society, 2009, 131, 5153-5162.
126、 Guo, R. T.,# Chong, Y. E.,# Guo, M., Yang, X. L.* Crystal structures and biochemical analyses suggest a unique mechanism and role for human glycyl-tRNA synthetase in Ap4A homeostasis. The Journal of Biological Chemistry, 2009, 284, 28968-28976.
127、 Chen, C. K., Lee, G. C., Ko, T. P., Guo, R. T., Huang, L. M., Liu, H. J., Ho, Y. F., Shaw, J. F., Wang, A. H.* Structure of the alkalohyperthermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding. Journal of Molecular Biology, 2009, 390, 672-685.
128、 Wu, Y. H., Ko, T. P., Guo, R. T., Hu, S. M., Chuang, L. M., Wang, A. H.* Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2. Structure, 2008, 16, 1714-1723.
129、 Kuo, C. J., Guo, R. T., Lu, I. L., Liu, H. G., Wu, S. Y., Ko, T. P., Wang, A. H., Liang, P. H.* Structure-based inhibitors exhibit differential activities against Helicobacter pylori and Escherichia coli undecaprenyl pyrophosphate synthases. Journal of Biomedicine & Biotechnology, 2008, 841312.
130、 Jeng, W. Y., Ko, T. P., Liu, C. I., Guo, R. T., Liu, C. L., Shr, H. L., Wang, A. H.* Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic Acids Research, 2008, 36, 1567-1577.
131、 Cao, R., Chen, C. K., Guo, R. T., Wang, A. H.,* Oldfield, E.* Structures of a potent phenylalkyl bisphosphonate inhibitor bound to farnesyl and geranylgeranyl diphosphate synthases. Proteins, 2008, 73, 431-439.
131、 C, K. M. C., Hudock, M. P., Zhang, Y., Guo, R. T., Cao, R., No, J. H., Liang, P. H., Ko, T. P., Chang, T. H., Chang, S. C., Song, Y., Axelson, J., Kumar, A., Wang, A. H., Oldfield, E.* Inhibition of geranylgeranyl diphosphate synthase by bisphosphonates: a crystallographic and computational investigation. Journal of Medicinal Chemistry, 2008, 51, 5594-5607.
133、 Guo, R. T.,# Cao, R.,# Liang, P. H., Ko, T. P., Chang, T. H., Hudock, M. P., Jeng, W. Y., Chen, C. K., Zhang, Y., Song, Y., Kuo, C. J., Yin, F., Oldfield, E., Wang, A. H.* Bisphosphonates target multiple sites in both cis- and trans-prenyltransferases. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104, 10022-10027.
134、 Chang, T. H., Guo, R. T., Ko, T. P., Wang, A. H.,* Liang, P. H.* Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination. The Journal of Biological Chemistry, 2006, 281, 14991-15000.
135、 Sun, H. Y., Ko, T. P., Kuo, C. J., Guo, R. T., Chou, C. C., Liang, P. H.,* Wang, A. H.* Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures. Journal of Bacteriology, 2005, 187, 8137-8148.
136、 Guo, R. T.,# Ko, T. P.,# Chen, A. P., Kuo, C. J., Wang, A. H.,* Liang, P. H.* Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis. The Journal of Biological Chemistry, 2005, 280, 20762-20774.
137、Guo, R. T.,# Kuo, C. J.,# Ko, T. P., Chou, C. C., Liang, P. H.,* Wang, A. H.* A molecular ruler for chain elongation catalyzed by octaprenyl pyrophosphate synthase and its structure-based engineering to produce unprecedented long chain trans-prenyl products. Biochemistry, 2004, 43, 7678-7686.
138、 Guo, R. T., Kuo, C. J., Chou, C. C., Ko, T. P., Shr, H. L., Liang, P. H.,* Wang, A. H.* Crystal structure of octaprenyl pyrophosphate synthase from hyperthermophilic Thermotoga maritima and mechanism of product chain length determination. The Journal of Biological Chemistry, 2004, 279, 4903-4912.
139、 Chu, H. M.,#Guo, R. T.,# Lin, T. W., Chou, C. C., Shr, H. L., Lai, H. L., Tang, T. Y., Cheng, K. J., Selinger, B. L., Wang, A. H.* Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis. Structure, 2004, 12, 2015-2024.
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已授权专利
1. 郭瑞庭，郑雅珊，吴姿慧，黄建文，赖惠琳，林正言。一种纤维素酶及其应用，授权号：ZL2011100426000，中国专利。
2. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言。酶活性及热稳定性被提高的葡聚糖酶，授权号：ZL2011100596815，中国专利。
3. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。提升酶活性之木聚糖酶，ZL2013101344011，中国专利。
4. 郭瑞庭，黄建文，郑雅珊，吴姿慧，赖惠琳，林正言，黄婷沅。酶活性提高的甘露聚糖酶，授权号：ZL201310135208X，中国专利。
5. 郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN104342418B，中国专利。
6. 郭瑞庭，陈纯琪，吴姿慧，郑雅珊，黄建文，赖惠琳，林正言，黄婷沅。具有提升的酶活性的植酸酶，授权号：CN106399273B，中国专利。
7. Guo, R. T., Zheng, Y. Y., Xu, Z. X., Chen, C. C., Peng, W., Liu, W. D., Liu, J. R., Ma, Y. H. Lactone hydrolase and method of degrading alpha-zearalenol using the same，授权号：US10316303B2，美国专利。
8. Guo, R. T., Cheng, Y. S., Wu, T. H., Huang, J. W., Lai, H. L., Lin, C. Y.，Thermostable cellulase having increased enzyme activity，授权号US8137945B1，美国专利。
9. Guo, R. T., Huang, J. W., Cheng, Y. S., Wu, T. H., Lai, H. L., Lin C. Y.，Glucanase having increased enzyme activity and thermostability，授权号US8192974B1，美国专利。
10. Guo, R. T., Cheng, Y. S., Huang, J. W., Wu, T. H., Lai, H. L., Lin, C. Y., Huang, T. Y，Xylanase having improved enzymatic activity，授权号US8722382B1，美国专利。
11. Guo, R. T., Huang, J. W., Cheng, Y. S., Wu, T. H., Lai, H. L., Lin, C. Y., Huang, T.Y，Beta-mannanase having improved enzymatic activity，授权号US8865444B1，美国专利。
12. Guo, R. T., Huang, J.W., Cheng, Y.S., Wu, T.H., Lai, H.L., Lin C.Y., Huang T. Y. β-mannanase having improved production yield and enzymatic activity，授权号US8999695B1，美国专利。
13. Guo, R. T., Wu, T. H., Cheng, Y. S., Huang, J. W., Lai, H. L., Lin C. Y., Huang T. Y. Phytase having improved enzymatic activity，授权号US9194010B2，美国专利。
14. Chen, C. C., Huang, J. W., Dai, L. H., Yu, X. J., Chen, C. Y., Wu, S., Zhan, Z. C., Zhang, L. L., Zhai, C., Ma, L. X., Guo, R. T. Cellulase having improved enzymatic activity，授权号：US10479985B1，美国专利。
15. Guo, R. T., Chen, C. C., Cheng, Y. S., Huang, J. W., Wu, T. H., Lai, H. L., Lin, C. Y., Ko, T Y. Cellulase having improved enzymatic activity，授权号：US9809807B1，美国专利。
16. Wu, T. H., Cheng, Y. S., Lai, H. L., Ke, T. Y., Huang, J. W., Chen, C .C., Guo, R. T. Phytase having improved thermostability，授权号：US10011822B2，美国专利。
17. Cheng, Y. S., Wu, T. H., Lin, C. Y., Lai, H. L., Zheng, C. B., Huang, T. Y., Lin, I. H., Huang, J. W., Chen, C. C., Guo, R. T. Glucose oxidase having improved thermostability，授权号：US10233430B1，美国专利。
18. Chen, C. C., Wang, T., Huang, J. W., Guo, R. T. Lysozyme having improved enzymatic activity，授权号：US10323237B2，美国专利。
19. Cheng, Y. S., Wu, T. H., Lin, C. Y., Lai, H. L., Zheng, C. B., Huang, T. Y., Lin, I. H., Huang, J. W., Chen, C. C., Guo, R. T. Xylosidase having improved enzymatic activity，授权号：US10385346B2，美国专利。
2021年以湖北大学图书馆为背景发表的P450综述文章
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