一、基本信息

李圣纯，博士，教授，博士生导师

电子邮件：shengchun_li(at)163.com

研究领域：质体（叶绿体）基因工程，植物氧化还原信号转导

学术兼职：湖北省植物生理与分子生物学学会第十一届理事会常务理事

社会兼职：九三学社湖北省第八届委员会委员，九三学社湖北大学委员会主任委员

二、教育背景

2009.03–2013.07  法国巴黎十一大学植物研究所，理学博士

2006.09–2008.07  华中科技大学生命科学与技术学院，理学硕士

2002.09–2006.07  武汉轻工大学生命科学与技术学院，工学学士

三、工作经历

2015.07至今         湖北大学生命科学学院，教师

2013.10–2014.06  中国农业科学院作物科学研究所，访问研究

四、主持项目

（一）科研项目

1. 国家自然科学基金面上基金（32071477）：氧化应激增强质体介导RNAi抗棉铃虫效果的分子机制及应用研究，2021.01–2024.12

2. 湖北省重点研发计划：油茬稻稻田低碳栽培及稻米品质检测关键技术研发，2021.01–2023.12

3. 国家自然科学基金青年基金（31700227）：PP2A-B'γ在过氧化氢诱导的细胞死亡中的机理研究，2018.01–2020.12，主持

4. 湖北省人才项目，2017.07–2022.06，主持

（二）教研项目

1. 湖北大学教学研究项目：研究生“课程思政”示范课程（《农业科技与政策》）项目，2024.01–2024.12

2. 湖北大学教学研究项目：课程思政在《遗传学》教学中的实施路径探索，2022.09–2024.08

五、发表论文（#共同第一作者，*通讯作者）

（一）第一或通讯作者科研论文

1. Chen Q, Wu Y, Wang Y, Zhang J^*, Li S^*. (2024) Stable plastid transformation of woody fruit tree kiwifruit (Actinidia chinensis). aBIOTECH

2. Li P^#, Song W^#, Zhang Y, Yang Y, Li S^*, Zhang J^*. (2024) Effects of drought stress on plastid-mediated RNA interference for controlling pest. Pesticide Biochemistry and Physiology 24, 106037.

3. Li S^*. (2023) Novel insight into functions of ascorbate peroxidase in plants: more than a simple antioxidant enzyme. Redox Biology 64, 102789.

4. Li S, Kim DS, Zhang J. (2023) Plastid-mediated RNA interference: a potential strategy for efficient insect pest control. Plant Cell & Environment 46, 2595–2605.

5. Yang S, Deng Y, Li S^*. (2022) Advances in plastid transformation for metabolic engineering in higher plants. aBIOTECH 3, 224–232.

6. Li S, Shen P, Wang B, Tian M, Chen T, Han Y. (2022) Modification of chloroplastic antioxidant capacity by plastid transformation technology in tobacco. Methods in Molecular Biology 2526, 3–13.

7. Chen Q, Shen P, Bock R, Li S^*, Zhang J^*. (2022) Comprehensive analysis of plastid gene expression during fruit development and ripening of kiwifruit. Plant Cell Reports 41, 1103–1114.

8. Li S^*, Ding H, Deng Y, Zhang J. (2021) Knockdown of quinolinate phosphoribosyltransferase results in decreased salicylic acid -mediated pathogen resistance in Arabidopsis thaliana. International Journal of Molecular Science 22, 8484.

9. Li S, Chang L, Zhang J. (2021) Advancing organelle genome transformation and editing for crop improvement. Plant Communications 2, 100141.

10. Ding H, Wang B, Han Y, Li S^*. (2020) Dehydroascorbate reductase, a pivotal regulator in ascorbate-glutathione pathway in plants. Journal of Experimental Botany 71, 3405–3416.

11. Zhang Y, Xu L, Li S^*, Zhang J^*. (2019) Bacteria-mediated RNA interference for management of Plagiodera versicolora (Coleoptera: Chrysomelidae). Insects 10, 415.

12. Wang B, Ding H, Chen Q, Ouyang L, Li S^*, Zhang J^*. (2019) Enhanced tolerance to methyl viologen-mediated oxidative stress via AtGR2 expression from chloroplast genome. Frontiers in Plant Science 10, 1178.

13. Chen Q, Wang B, Ding H, Zhang J^*, Li S^*. (2019) The role of NADP-malic enzyme in plants under stress. Plant Science 281, 206–212.

14. Rahantaniaina MS^#, Li S^#, Chatel-Innocenti G, Andrée Tuzet A, Issakidis-Bourguet E, Mhamdi A, Noctor G. (2017) Chloroplastic and cytosolic dehydroascorbate reductases co-operate in oxidative stress-driven activation of the salicylic acid pathway. Plant Physiology 174, 956–971.

15. Li S, Mhamdi A, Trotta A, Kangasjärvi S, Noctor G (2014) The protein phosphatase subunit PP2A-B'γ is required to suppress daylength-dependent pathogenesis responses triggered by intracellular oxidative stress. New Phytologist 202, 145–160.

16. Li S^#, Mhamdi A^#, Dizengremel P, Jolivet Y, Noctor G. (2013) Analysis of knockout mutants suggests that Arabidopsis NADP-MALIC ENZYME2 does not play an essential role in determining phenotypes or regulating redox state in response to oxidative stress of intracellular or extracellular origin. Journal of Experimental Botany 64, 3605–3614.

17. 吴梦婷，王海涛，张淼，许文博，张望，李圣纯^*.（2022）利用植物质体转基因技术高效表达抗人源白介素6单链抗体. 生物工程学报 38，2269-2280.

（二）  合作作者科研论文

1. Jiang C, Fu J, Xia K, Li S, Chang L, Zhang J. (2024) Plastid engineering with an efficient RNAi delivery system based on bacteriophage MS2 virus-like particle enhances resistance to Helicoverpa armigera. Molecular Plant 17: 987–989.

2. Xu W, Li S, Bock R, Zhang J. (2024) A heat-inducible expression system for external control of gene expression in plastids. Plant Biotechnology Journal 22, 960–969.

3. Li Y, Xu H, He W, Rong H, Li S, Kim D, Han P, Yang Y, Zhang J. (2023) Silencing of insect dsRNase genes enhances the plastid-mediated RNAi effect on the Colorado potato beetle. Entomologia Generalis 43, 69–77.

4. Wu M, Zhang Q, Dong Y, Wang Z, Zhan W, Ke Z, Li S, He L, Ruf S, Bock R, Zhang J. (2023) Transplastomic tomatoes expressing double-stranded RNA against a conserved gene are efficiently protected from multiple spider mites. New Phytologist 237, 1363–1373.

5. Xu W, Zhang M, Li Y, He W, Li S, Zhang J. (2023) Complete protection from Henosepilachna vigintioctopunctata by expressing long double-stranded RNAs in potato plastid. Journal of Integrative Plant Biology 65, 1003–1011

6. Dong Y, Wu M, Zhang Q, Fu J, Loiacono FV, Yang Y, Wang Z, Li S, Chang L, Bock R, Zhang J. (2022) Control of a sap-sucking insect pest by plastid-mediated RNA interference. Molecular Plant 15, 1176–1191.

7. Wu M, Dong Y, Zhang Q, Li S, Chang L, Loiacono FV, Ruf S, Zhang J, Bock R. (2022) Efficient control of western flower thrips by plastid-mediated RNA interference. Proceedings of the National Academy of Sciences of the United States of America 119, e2120081119.

8. Fu J, Xu S, Lu H, Li F, Li S, Chang L, Heckel DG, Bock R, Zhang J. (2022) Resistance to RNA interference by plant-derived double-stranded RNAs but not plant-derived short interfering RNAs in Helicoverpa armigera. Plant, Cell & Environment 45, 1930–1941.

9. Zhang T, Ma M, Chen T, Zhang L, Fan L, Zhang W, Wei B, Zhang D, Li S, Xuan W, Noctor G, Han Y. (2020) Arabidopsis S-nitrosoglutathione reductase1 coordinates glutathione-mediated activation of salicylic acid pathway in response to intracellular oxidative stress. Plant Cell & Environment 43, 1175–1191.

10. Durian G, Jeschke V, Vuorinen K, Rahikainen M, Gollan P, Glawischnig E, Winter Z, Li S, Noctor G, Aro EM, Kangasjärvi J, Overmyer K, Burow M, Kangasjärvi S. (2020) Protein phosphatase 2A-B′γ controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiology 182, 1161–1181.

（三）教学论文

1. 李海涛，李圣纯^*.（2024）新时代背景下遗传学课程教学改革探索. 创新教育研究 12, 523–528.

2. 李圣纯^*，王家琦，陈楚铭.（2023）文献导读在细胞工程教学中的实践. 科教导刊 31, 48–50.

3. 李圣纯*，刘会.（2022）遗传学教学中若干问题的讨论. 生物学通报57, 36–38.

4. 李圣纯*，张江.（2021）《细胞工程》教学中融合思政教育的探索. 教育现代化 8, 172–174.

六、指导学生情况

1. 2024年，国际基因工程机器大赛（iGEM），银奖（第一导师）

2. 2024年，第三届合成生物学创新赛，最佳答辩奖（第一导师）

3. 2024年，“挑战杯”大学生创业计划竞赛，省赛金奖、国赛铜奖（第一导师）

4. 2024年，中国国际大学生创新大赛，省赛金奖、国赛铜奖（第一导师）

5. 2023年，中国国际大学生创新大赛，省赛金奖、国赛铜奖（第一导师）

6. 2023年，第二届中非青年创新创业大赛，第一名（第一导师）

7. 2023年，2019级生物技术国重班考研升学率81%（全校第一，班主任）

8. 2020年，2018级硕士丁海燕获国家奖学金

9. 2019年，2017级硕士王必鹏获国家奖学金

七、荣誉

1. 2020~2022年度，湖北大学优秀教师

2. 2016年，入选湖北省省级青年人才