姓 名:李珊珊

办公电话:027-88661237-8082

电子邮件:shanshanyxl@hotmail.com; shl@hubu.edu.cn

通讯地址:湖北省武汉市武昌区友谊大道368号湖北大学生命科学学院B204室

任职情况：博士，现任湖北大学教授、博士研究生导师

社会兼职：中国细胞生物学学会染色质生物学分会委员，中国老年学和老年医学学会老年病分会衰老基础医学专家委员会常务委员，中国生物化学与分子生物学教学青年委员会委员，湖北省青年科技工作者常务理事

研究领域：细胞代谢与表观遗传修饰在肿瘤与衰老中的作用

肿瘤细胞在细胞代谢方面与正常细胞有着重大区别：正常细胞利用氧化磷酸化高效产生能量，而癌细胞却依赖有氧糖酵解而不是氧化磷酸化来产生能量。许多参与糖酵解的酶在癌细胞中特异性表达而且在癌细胞的生长增殖中起关键作用，已经成为肿瘤治疗的潜在靶位点。针对代谢酶开发的肿瘤治疗方法可以克服普通化疗产生的抗药性、促进化疗引起的癌细胞凋亡。然而，由于大多数代谢酶在肿瘤发生中的作用机制尚不完全清楚，肿瘤代谢治疗方法在应用方面受到了极大的限制。

近来研究表明细胞代谢包括代谢酶和代谢产物可以直接调控表观遗传修饰，比如DNA甲基化、组蛋白乙酰化、组蛋白甲基化等。表观遗传修饰在肿瘤发生、细胞衰老中起重要作用。表观遗传改变在理论上来讲是可逆的，因此有利于开发抗肿瘤和抗衰老的药物。为全面了解代谢酶的功能，本实验室主要以从下述三个方向进行研究：

1、细胞代谢酶以及代谢产物在肿瘤发生中的作用以及表观遗传调控机制。

2、细胞代谢酶以及代谢产物在衰老中的作用以及表观遗传调控机制。

3、抗肿瘤、抗衰老药物筛选与化学干预。

一、学习和工作简历

2000/09–2004/06，华中农业大学，生命科学技术学院，学士

2004/09–2006/06，武汉大学，微生物学，硕士

2006/08–2010/12，美国爱荷华州立大学，分子细胞发育生物学，博士

2011/01–2013/08，美国爱荷华州立大学，微生物学，博士后

2013/08–2016/01，美国斯托瓦斯医学研究所，博士后

2016/02-至今，湖北大学，生命科学学院，教授

二、承担科研项目情况

1.国家自然科学基金，丙酮酸通过抑制组蛋白表达调控肿瘤细胞生长的机制研究；2020.1-2023.12，主持。

2.国家自然科学基金（31673115）：丙酮酸激酶调控端粒异染色质基因沉默的机制研究；2017.1-2020.12，主持。

3.湖北省自然科学基金“杰青”项目（2017CFA066）：二甲双胍抗肿瘤的表观遗传调控的机制研究；2017.1-2019.12，主持。

4.生物资源绿色转化湖北省协同创新中心专项启动经费，2015.12-2018.12，主持。

5.中国国家自然科学基金，31460667，乙脑病毒激活内质网应激反应的分子机制及致病性研究，2015/01-2018/12，参与。

6.中国国家自然科学基金，31160034，内质网应激反应和丙肝病毒NS4B致病性研究，2011/01-2015/12，已结题，参与。

7.美国国家自然科学基金，MCB-0920156，Cellular responses to quaternary ammonium compounds byPseudomonas syringaethat influence its interactions with plants，2011/01-2013/12，已结题，参与。

8.美国国立卫生院基金，GM79663，Histone Modifications and Higher-Order Chromatin Structure，2007/01-2010/12，已结题，参与。

三、发表论文（*通讯作者）

1.Shanshan Li, Selene K. Swanson, Madelaine Gogol, Laurence Florens, Michael P. Washburn, Jerry L. Workman, Tamaki Suganuma. Serine and SAM responsive complex SESAME regulates histone modification crosstalk by sensing cellular metabolism.Mol Cell2015, 60(3):408-21.

2.Qianyun Mei, Chen Xu, Gogol Madelaine, Jie Tang, Wanping Chen, Xilan Yu, Jerry L. Workman*,Shanshan Li*. Set1-catalyzed H3K4 trimethylation antagonizes theHIR/Asf1/Rtt106 repressor complex to promotehistone gene expression and chronological life span.Nucleic Acids Res.2019, 47(7):3434-3449.

3.Rui Ma^#, Yinsheng Wu^#, Yansheng Zhai,Bicheng Hu,WeiMa,WenqiangYang,Qi Yu,Zhen Chen, Jerry L. Workman,Xilan Yu*,Shanshan Li*.Exogenous pyruvaterepresseshistonegeneexpressionand inhibits cancer cell proliferation via theNAMPT-NAD⁺-SIRT1pathway.Nucleic Acids Res.2019,47(21): 11132-11150.

4.Shanshan Liand Michael A. Shogren-Knaak*. Cross-talk between histone H3 tails produces cooperative nucleosome acetylation.Proc Natl Acad Sci U S A2008, 105(47):18243-18248.

5.Xilan Yu, andShanshan Li*.Non-metabolic functions of glycolytic enzymes intumorigenesis.Oncogene2017, 36(19):2629-2636.

6.Shanshan Li,and Michael Shogren-Knaak, A. The Gcn5 bromodomain of the SAGA complex facilitates cooperative and cross-tail acetylation of nucleosomes.J Biol Chem.2009, 284(14), 9411-9417.

7.Xilan Yu, Rui Ma, Yinsheng Wu, Yansheng Zhai,Shanshan Li*. Reciprocal regulation of metabolic reprogramming and epigenetic modifications in cancer.Frontiers in Genetics2018,9:394.

8.Qianruo Wang, Xin Xiu, Wang Ting, Wan Jiawu, Ou Yangtao, Yang Zibing, Yu Qijia, Zhu Liting, Guo Yunli, Wu Yinsheng, Ding Zhen, Zhang Yanni, Pan Zishu, Tang Yuxin,Shanshan Li*,LingbaoKong*. Japanese encephalitis virus induces apoptosis and encephalitis by activating the PERK pathway.J Virol2019, 93:e00887-19.

9.Shanshan Li*, Lingbao Kong, Xilan Yu. The expanding roles of endoplasmic reticulum stress in virus replication and pathogenesis.Crit Rev Microbiol.2015,41(2): 150-64.

10.Lingbao Kong^#,*,Shanshan Li^#,*, et al. Oleanolic acid and ursolic acid: novel Hepatitis C virus antivirals that inhibit NS5B activity.Antiviral Res.2013, 98(1), 44-53.

11.Shanshan Li, Lingbao Kong, Xilan Yu, Yi Zheng. Host-virus interactions: From the perspectives of epigenetics.Rev Med Virol.2014, 24(4): 223-41.

12.Yi Zheng, Bicheng Hu, Shenggao Xie, Xiaofan Chen, Yuqian Hu, Wanping Chen,Shanshan Li*, Bo Hu*. Dendritic cells infected by Ad-sh-SOCS1 enhance cytokine-inducedkiller (CIK) cell immunotherapeutic efficacy in cervical cancer models.Cytotherapy2017, 19: 617-628.

13.Qi Yu, Chong Tong, Mingdan Luo, Xiangyan Xue, Qianyun Mei, Lixin Ma, Xiaolan Yu, Wuxiang Mao, Lingbao Kong, Xilan Yu*,Shanshan Li*. (2017) Regulation of SESAME-mediated H3T11 phosphorylation by glycolytic enzymes and metabolites.PLoS One, 12(4):e0175576.

14.Shanshan Li, Linbai Ye, Xilan Yu, Xu B, Li K, Zhu X, Liu H, Wu X, Kong L.Hepatitis C virus NS4B induces unfolded protein response and endoplasmic reticulum overload response-dependent NF-κB activation.Virology.2009, 391(2): 257-64.

15.Fenglin Guo, Xilan Yu, Ahui Xu, Jing Xu, Qianruo Wang, Yunli Guo, Xiaoyu Wu, Yuxin Tang, Zhen Ding, Yanni Zhang, Tian Gong, Zishu Pan,Shanshan Li*, Lingbao Kong*.(2018)Japanese encephalitis virus induces apoptosis by inhibiting Foxo signaling pathway.Veterinary Microbiology, 220: 73-82.

16.LingbaoKong^#,Shanshan Li^#,XilanYu,XiaonanFang,AhuiXu,MingjieHuang,XiaoyuWu,YunliGuo,FengliGuo,JinXu.(2016)Hepatitis C virus and its protein NS4B activate the cancer-related STAT3 pathway via the endoplasmic reticulum overload response.Archives of Virology,161(8): 2149-2159

17.Chiliang Chen,Shanshan Li, Dana R. McKeever D, Gwyn A. Beattie.(2013)The widespread plant-colonizing bacterial speciesPseudomonas syringaedetects and exploits an extracellular pool of choline in hosts.ThePlant Journal,75(6): 891-902.

18.Lixin Qiu, Bicheng Hu, Hongbo Chen,Shanshan Li, Yuqian Hu, Yi Zheng, Xinxing Wu. (2015)Antifungal efficacy of itraconazole-loaded TPGS-b-(PCL-ran-PGA) nanoparticles.InternationalJournal of Nanomedicine,10:1415-23.

19.Yongqiang Zhu,YiZheng,LinMei,MengqiangLiu,Shanshan Li,HuaweiXiao,HuijunZhu,ShuWu,HongboChen,LaiqiangHuang.(2013)Enhanced immunotherapeutic effect of modified HPV16 E7-pulsed dendritic cell vaccine by an adeno-shRNA-SOCS1 virus.InternationalJournal of Oncology,43(4):1151-9.

20.XiaonanFang,LinbaiYe,Khalid AmineTimani,Shanshan Li,YingchunZen,MengZhao,HongZheng,ZhenghuiWu.(2005)Peptide domain involved in the interaction between membrane protein and nucleocapsid protein of SARS-associated coronavirus.Journal of Biochemistry &Molecular Biology, 38(4):381-385.

21.Fei Wang, Quanjiao Chen, Shuntang Li, ChenyaoZhang,Shanshan Li, Min Liu, Kun Mei, Chunhua Li, LixinMa, Xiaolan Yu. (2017)Linear DNA vaccine prepared by large-scale PCRprovides protective immunity against H1N1 influenza virusinfection in mice.Veterinary Microbiology,205:124-130.

22.LingbaoKong,Shanshan Li,XueHan,ZhonghuaXiang,XiaonanFang,BaizongLi,WeiWang,HongZheng,JinrongGao,LinbaiYe.(2007)Inhibition of HCV RNA-dependent RNA polymerase activity by aqueous extract from Fructus Ligustri Lucidi.Virus Research, 128(1):9-17.

23.BaozongLi,BoGao,LinbaiYe, XueHan,WeiWang,LingbaoKong,XiaonanFang,YingchunZeng,HongZheng,Shanshan Li,ZhenghuiWu,LinbaiYe.(2007)Hepatitis B virus X protein (HBx) activates ATF6 and IRE1-XBP1 pathways of unfolded protein response.Virus Research, 124(1-2):44-9.

24.XiaonanFang,LinbaiYe,YijuanZhang,BaozongLi,Shanshan Li,LingbaoKong,YuhuaWang,HongZheng,WeiWang,ZhenghuiWu.(2006)Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.The Journal of Microbiology, 44(5):577-80.

四、获奖

1、江西省自然科学奖三等奖，2016

2、江西省高校科技成果奖二等奖，2011

3、第二届全国大学生生命科学创新创业大赛指导奖一等奖，2017。

4、湖北大学五四青年奖章，2019。

五、专利

1.李珊珊、马瑞等。一种将白藜芦醇和丙酮酸组合用于抗肿瘤药物。专利申请号：201711156206.3。

2.李珊珊。一种用于检测组蛋白豆蔻酰化修饰的重组蛋白及其应用。专利授权号：201710147487X。