一、基本信息

余晓岚，博士，教授，博士研究生导师

办公电话：027–88661237

电子邮件：yxiaolan@163.com

社会兼职：中国中国微生物学会会员、湖北省微生物学会会员、湖北省免疫学

会理事、武汉市免疫学会理事

研究领域：动物新型疫苗，抗病毒多肽，抗体免疫学，病毒与宿主互作

二、教育背景

2000.09–2005.07  华中农业大学动物医学学院，农学博士

1996.09–2000.07  华中农业大学动物科学学院，农学学士

三、工作经历

2005.09至今          湖北大学生命科学学院讲师、副教授、教授

2015.02–2015.05  美国凯斯西储大学，博士后

2013.01–2014.01  美国凯斯西储大学，Associate Research

2012.03–2012.09  美国凯斯西储大学，访问学者

四、科研项目

1.     横向课题：抗体研制；2020.1–2023.12，200万，主持

2.     国家自然科学基金（31672561）：展示靶向单链抗体(scFv)的细菌膜影(ghost)递送线性DNA疫苗的分子机制研究；2017,1–2020,12，74万元，主持

3.     国家自然科学基金(31340036)：基于酿酒酵母靶向性递呈和表达的DNA疫苗研究；62万元，2014.1– 2014.12，主持

4.     国家自然科学基金（31172320）：基于大规模PCR技术高通量制备DNA疫苗方法的研究；19万元，2012.1–2015.12，主持

5.     国家自然科学基金（30801008）：SPOP与MyD88的相互作用调控TLR信号途径的分子机理研究；2009.1–2011.12，主持

6.     武汉市青科技晨光计划(201050231086）：基于家蚕重组杆状病毒的高效蛋白表达系统的研究；3万元，2010.1–2012.12，主持

7.     湖北省教育厅重点项目（D20111005）：基于大规模PCR技术线性DNA疫苗的研究；2011.1– 2012.12，主持

8.     湖北省教育厅–湖北省高等学校青教师深入企业行动计划项目（XD2010118）：猪用干扰素发酵中试放大研究，0.5万元，2011.1–2011.12，主持

9.     湖北省自然科学基金（2008CDB11）：SPOP与MyD88的相互作用调控TLR信号途径的研究，2万元，2009.1–2010.12，主持人。

10.  国家微生物重点实验室开放基金，AML–0801，基于口蹄疫病毒（FMDV）P1 DNA疫苗及牛疱疹病毒VP22蛋白的佐剂作用的研究，5万元，2008.9–2010.8，主持人。

五、代表性论文（^#共同一作，*通讯作者）：

1.      Wang B, Yang Z, Gao D, Wang F, Liu M, Chen G, Ma L, Yu X*. (2021) Design of fusion protein for efficient preparation of cyanovirin–n and rapid enrichment of pseudorabies virus. Biotechnology Letters, https://doi.org/10.1007/s10529–021–03141–x

2.      Yu X*, Ye F. (2020) Role of angiopoietins in development of cancer and neoplasia associated with viral infection. Cells 9, 457.

3.      Liu M, Wang B, Wang F, Yang Z, Gao D, Zhang C, Ma L, Yu X*. (2019) Soluble expression of single-chain variable fragment (scFv) in Escherichia coli using superfolder green fluorescent protein as fusion partner. Applied Microbiology and Biotechnology 103, 6071–6079.

4.      Wang F, Chen Q, Li S, Zhang C, Li S, Liu M, Mei K, Li C, Ma L, Yu X*. (2017) Linear DNA vaccine prepared by large-scale PCR provides protective immunity against H1N1 influenza virus infection in mice. Veterinary Microbiology 205, 124–130.

5.      Yu X, Sha J, Xiang S, Qin S, Conrad P, Ghosh SK, Weinberg A, Ye F. (2016) Suppression of KSHV-induced angiopoietin-2 inhibits angiogenesis, infiltration of inflammatory cells, and tumor growth. Cell Cycle 15, 2053–2065.

6.      Yu X, Shahir AM, Sha J, Feng Z, Eapen B, Nithianantham S, Das B, Karn J, Weinberg A, Bissada NF, Ye F. (2014) Short chain fatty acids from periodontal pathogens suppress HDACs, EZH2, and SUV39H1 to promote Kaposi's Sarcoma-associated herpesvirus replication. Journal of Virology 88, 4466–4479.

7.      Yu X, Mao W, Zhai Y, Tong C, Liu M, Ma L, Yu X*, Li S. (2017) Anti-tumor activity of metformin: from metabolic and epigenetic perspectives. Oncotarget 8, 5619–5628.

8.      Wang F, Li S, Hui Zhao H, Bian L, Chen L, Zhang Z, Zhong X, Ma L, Yu X*. (2015) Expression and characterization of the RKOD DNA polymerase in Pichia pastoris. PLoS One 10, e0131757.

9.      Mei K, Liu G, Chen Z, Gao Z, Zhao L, Jin Y, Yu X*, Chen Q. (2016) Deep sequencing reveals the viral adaptation process of environment-derived H10N8 in mice. Infection, Genetics and Evolution 7, 8–13.

10.   Meng Li^#, Yu X^#, Fei Wang F, Chao Zhai C, Wei Shen W, Xianhong Yu X, Xiaojuan Wang X, Ma L. (2015) Secretory expression of organophosphorus hydrolase OPHC2 in Yarrowia lipolytica Polg. Journal of Environmental Science and Health, Part B 50, 691–697.

11.   Yu X, Junqing Hua J, Guibin Hu G, Jie Zhang J, Ma L. (2014) Expression of recombinant protein in insect cells by their direct infection with Escherichia coli transformed with baculovirus bacmids. Acta Virologica 58, 61–68.

12.   Yu X, Xiao S, Fang L, Jiang Y, Chen H, Jin M. (2012) A DNA vaccine encoding the FMDV capsid precursor polypeptide P1 and the enhancing effect of bovine herpesvirus 1 VP22 protein as molecular adjuvant. Acta Virologica 56, 111–117.

13.   Bhaskaran N, Ghosh SK, Yu X, Qin S, Weinberg A, Pandiyan P, Ye F. (2017) Kaposi's sarcoma-associated herpesvirus infection promotes differentiation and polarization of monocytes into tumor-associated macrophages. Cell Cycle 16, 1611–1621.

14.   Yu Q, Tong C, Luo M, Xue X, Mei Q, Ma L, Yu X, Mao W, Kong L, Yu X, Li S. (2017) Regulation of SESAME-mediated H3T11 phosphorylation by glycolytic enzymes and metabolites. PLoS One 12, e0175576.

15.   Wang F, Wang X, Yu X, Fu L, Liu Y, Ma L, Zhai C. (2015) High-level expression of endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and its application for the deglycosylation of glycoproteins. PLoS One 10, e013792.

16.   Bi Y, Mei K, Shi W, Di Liu D, Yu X, Gao Z, Zhao L, Gao GF, Chen J, Chen Q. (2015) Two novel reassortants of avian influenza A (H5N6) virus in China. Journal of General Virology 96, 975–981.

17.   Das B, Dobrowolski C, Shahir AM, Feng Z, Yu X, Sha J, Bissada NF, Weinberg A, Karn J, Ye F. (2015) Short chain fatty acids potently induce latent HIV-1 in T-cells by activating P-TEFb and multiple histone modifications. Virology 474, 65–81.

18.   Zhong X, Zhai C, Yang D, Jiang S, Li Z, Yu X, Chen L, Zhang Z, Wang F, Wang Y, Chen W, Ma L. (2013) A single-step mixing cloning method for assembly of lentiviral short hairpin RNA expression vectors for gene silencing. Analytical Biochemistry 438, 39–41.

19.   Ye F, Zhou F, Nithianantham S, Chandran B, Yu X, Weinberg A, Gao S. (2013) Kaposi’s sarcoma-associated herpesvirus induces rapid release of angiopoietin-2 from endothelial cells. Journal of Virology 87, 6236–6335.

20.   Chen X, Zhai C, Kang L, Li C, Yan H, Zhou Y, Yu X, Ma L. (2012) High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris. Biotechnology Letter 34, 689–694.

21.   Yu X, Xiao S, Fang L, Jiang Y, Chen H. (2006) Enhanced immunogenicity to foot-and-mouth disease virus in mice vaccination with alphviral replicon-based DNA vaccine expressing the capsid precursor polypeptide (P1). Vrius Genes 33, 337–344.

22.   Fu J, Lin G, Zeng B, Wu Z, Wu Y, Chu H, Qin G, Liang G, Li J, Gan X, Yu X, Li C, Liu D. (2006) Anti-ischemia/reperfusion of C1 inhibitor in myocardial cell injury via regulation of local myocardial C3 activity. Biochemical and Biophysical Research Communications 350, 162–168.

23.   Jiang Y, Xiao S, Fang L, Yu X, Song Y, Niu C, Chen H. (2006) DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Vaccine 24, 2869–2879.

24.   Yan L, He Q, Yu X, Bei W, Chen H. (2005) The co-administrating of recombinant porcine IL-2 could enhance protective immune responses to PRV inactivated vaccine in pigs. Vaccine 23, 4436–4441.

六、专利

1.    重组CL7–CVN蛋白及其制备方法与应用，2021，中国，ZL201911203478.3

2.    一种耐高温壳聚糖酶酵母工程菌及其耐高温壳聚糖酶的生产方法，2013，中国，ZL201010507427.2

3.    一种高通量的通过细菌直接侵染将外源基因导入昆虫细胞的方法，2011，中国，ZL200710052967.4

4.    一种利用毕赤酵母高效表达DNA聚合酶的方法，2012.10，中国，ZL201010229160.5

5.    一种快速高校构建高效构建哺乳动物细胞RNA干扰载体及其验证载体的方法，2011，中国，ZL200710052912.3

6.    一种简单高效制备纯化TaqDNA聚合酶的方法，2012，中国，ZL201210165241.2

七、获奖

1.      第四届全国大学生生命科学创新创业大赛二等奖，指导老师，2019，教育部

2.      湖北省自然科学二等奖，2018，湖北省人民政府

3.      湖北省科学技术发明奖二等奖，2016，湖北省人民政府

4.      第四届湖北省高等学校大学生生物实验技能竞赛一等奖，指导老师，2016，湖北省教育厅

5.      指导学生获得湖北省优秀学士学位论文，2012.12，湖北省教育厅

八、人才荣誉

1.      2018，武汉市光谷人才计划