杨世辉，博士，湖北大学生命科学学院教授，博士生导师，主要从事微生物代谢工程、合成生物学、系统生物学以及生物能源及重大平台化合物的绿色生物制造等方面的研究，先后在Nature Biotechnology, Science Advances及PNAS等期刊发表论文 48篇，引用1700多次，H-index 23。担任国际学术期刊PLoS ONE 学术编辑，Energies 编辑，Frontiers in Microbiology 主题编辑，Frontiers in Energy Research及Frontiers in Bioengineering and Biotechnology副编辑；Science, PNAS, PLoS Genetics等学术杂志审稿人；多次应邀在国际学术会议上做大会报告并作为会议主持人。
Prof. YANG received Ph.D. degree in microbiology from University of California at Riverside in 2005, and his research interest focuses on metabolic engineering, synthetic biology, and renewable bioproducts. He has published 49 papers in prestigious journals including Nature Biotechnology and PNAS with a citation more than 1700 and H-index of 23. He also serves the scientific community as an editor of international journals such as PLoS ONE, Energies, Frontiers in Microbiology, Frontiers in Bioengineering and Biotechnology, and Frontiers in Energy Research; and a reviewer for journals like Science, PNAS, PLoS Genetics, and Biotechnology for Biofuels.
Biorefinery
of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative
of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen
with many desirable characteristics, which makes it an ideal industrial microbial
biocatalyst for commercial production of desirable bioproducts through metabolic
engineering.
In this study,
we determined the complete chromosome and plasmid sequences of ZM4 and its engineered
xylose-utilizing derivatives 2032 and 8b. Compared to previously published and revised
ZM4 chromosome sequences, the ZM4 chromosome sequence reported here contains 65
nucleotide sequence variations as well as a 2400-bp insertion.
Pretreatment
is the key step to overcome the recalcitrance of lignocellulosic biomass making
sugars available for subsequent enzymatic hydrolysis and microbial fermentation.
During the process of pretreatment and enzymatic hydrolysis as well as fermentation,
various toxic compounds may be generated with strong inhibition on cell growth and
the metabolic capacity of fermenting strains.
The aim of this
work was to identify inhibitors in pretreated lignocellulosic slurries, evaluate
high-throughput screening strategies, and investigate the impact of inhibitors on
potential hydrocarbon-producing microorganisms. Compounds present in slurries that
could inhibit microbial growth were identified through a detailed analysis of saccharified
slurries by applying a combination of approaches of high-performance liquid chromatography,
GC-MS, LC-DAD-MS, and ICP-MS.
