副教授

赵晶

作者:生命科学学院发布时间:2019年08月01日 00:00

一、基本信息

赵晶,博士,副教授、硕士研究生导师

办公电话:15522275019

电子邮件:zhaojing@hubu.edu.cn, zhaojing802@hotmail.com

研究领域:计算生物学、酶工程和生物催化

二、教育背景

2011.102015.09  德国亚琛工业大学,理学博士

2008.092011.06  华东理工大学,工学硕士

2004.092008.07  南京农业大学,工学学士

三、工作经历

2019.07至今      湖北大学生命科学学院,副教授

2019.012019.07  湖北大学生命科学学院,讲师

2015.122018.12  中国科学院天津工业生物技术研究所,助理研究员

四、科研项目

1.      国家自然科学基金(21606251):赖氨酸脱羧酶多聚体界面氨基酸对酶活性和最适pH影响规律的研究;2017.01-2019.12,主持

2.      天津市自然科学基金(18JCQNJC10300):手性砌块反式-4-羟基-L-脯氨酸生物法绿色制造关键酶的多目标分子进化与应用研究;2018.04-2021.03,主持

3.      天津市留学人员科技活动启动项目择优资助(2017年度):同时进化酶的可溶性和活性获得新型赖氨酸脱羧酶的研究,2018.01-2018.12,主持

4.      国家自然科学基金(31870081):以异亮氨酰-tRNA合成酶为例构建一类新型氨基酸生物传感器;2019.01-2022.12,参与

代表性文章(#共同一作,*通讯作者):

1.      Zhao J#, Liu C#, Guo X#, Wang J, Liu H, Zheng P, Sun J, Ma Y. (2019) Efficient production of trans-3-hydroxyproline by a bacterial trans-3-proline hydroxylase and characterization of enzymatic properties. Biochemical Engineering Journal 147, 57–61.

2.      Liu C#, Zhao J#, Liu J, Guo X, Rao D, Liu H, Zheng P, Sun J, Ma Y. (2019) Simultaneously improving the activity and thermostability of a new proline 4-hydroxylase by loop grafting and site-directed mutagenesis. Applied Microbiology and Biotechnology 103, 265–277.

3.      Zhao J, Frauenkron-Machedjou VJ, Fulton A, Zhu L, Davari MD, Jaeger KE, Schwaneberg U, Bocola M. (2018) Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study, Physical Chemistry Chemical Physics 20, 9600–9609.

4.      Zhao J, Frauenkron-Machedjou VJ, Kardashliev T, Ruff AJ, Zhu L, Bocola M, Schwaneberg U. (2017) Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations. Applied Microbiology and Biotechnology 101, 3177–3187.

5.      Zhao J, Jia N, Jaeger KE, Bocola M, Schwaneberg U. (2015) Ionic liquid activated Bacillus Subtilis lipase A variants through cooperative surface substitutions. Biotechnology and Bioengineering 2015, 112, 1997–2004.

6.      Zhao J, Kardashliev T, Ruff AJ, Bocola M, Schwaneberg U. (2014) Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations. Biotechnology and Bioengineering 111, 2380–2389.

7.      Zhao J, Chu Y, Li A, Ju X, Kong X, Pan J, Tang Y, Xu J. (20) An unusual (R)-selective epoxide hydrolase with high activity for facile preparation of enantiopure glycidyl ethers. Advanced Synthesis & Catalysis, 2011, 353 (9): 1510–1518.

8.      Kou F#, Zhao J#, Liu J, Shen J, Ye Q, Zheng P, Li Z*, Sun J*, Ma Y. (2016) Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH. Journal of Molecular Catalysis B: Enzymatic 133(Supplement 1), S88–S94.

9.      Tan Z#, Zhao J#, Chen J, Rao D, Zhou W, Chen N, Zheng P*, Sun J, Ma Y. (2019) Enhancing thermostability and removing hemin inhibition of Rhodopseudomonas palustris 5-aminolevulinic acid synthase by computer-aided rational design. Biotechnology Letters  41, 181–191.

10.   Xu D#, Zhao J#, Cao G, Wang J, Li Q, Zheng P, Zhao S, Sun J. (20) Removal of feedback inhibition of Corynebacterium glutamicum phosphoenolpyruvate carboxylase by addition of a short terminal peptide. Biotechnology and Bioprocess Engineering, 2018, 23(1): 72–78.

11.   Liu J, Wang Y, Lu Y, Ni X, Guo X, Zhao J, Chen J, Dele-Osibanjo T, Zheng P, Sun J, Ma Y. (2018) Mutations on peptidoglycan synthesis gene ponA improve electro-transformation efficiency of Corynebacterium glutamicum ATCC 13869, Applied and Environmental Microbiology 84, e02225-18.

12.   Li R, Li A, Zhao J, Chen Q, Li N, Yu H, Xu J. (2018) Engineering P450LaMO stereospecificity and product selectivity for selective C-H oxidation of tetralin-like alkylbenzenes. Catalysis Science & Technology 8, 4638–4644.

13.   Xu G, Wang Y, Tang M, Zhou J, Zhao J, Han R, Ni Y. (2018) Hydroclassified combinatorial saturation mutagenesis: reshaping substrate binding pockets of KpADH for enantioselective reduction of bulky-bulky ketones. ACS Catalysis 8, 8336–8345.

14.   Li R, Xu J, Chen Q, Zhao J, Li A, Yu H. (20) Enhancing the catalytic performance of a CYP116B monooxygenase by transdomain combination mutagenesis. ChemCatChem, 2018, 10(14): 2962–2968.

15.   Wang X#, Liu J#, Zhao J, Ni X, Zheng P, Guo X, Sun C, Sun J, Ma Y. (2018) Efficient production of trans-4-hydroxy-L-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. Journal of Bioscience and Bioengineering, 2018, 126(4): 470–477.

16.   Kou F, Zhao J, Liu J, Sun C, Guo Y, Tan Z, Cheng F, Li Z, Zheng P, Sun J. (2018) Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production. Biotechnology Letters 40, 719–727.

17.   Markel U, Zhu L, Frauenkron-Machedjou VJ, Zhao J, Bocola M, Davari MD, Jaeger KE, Schwaneberg U. (2017) Are directed evolution approaches efficient in exploring nature’s potential to stabilize a lipase in organic cosolvents? Catalysts 7, 142.

18.   Cao H, Li C, Zhao J, Wang F, Tan T, Liu L. (2018) Enzymatic production of glutathione coupling with an ATP regeneration system based on polyphosphate kinase. Applied Biochemistry and Biotechnology 185, 385–395.

19.   Liu X, Xu J, Pan J, Zhao J. (2010) Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01. Applied Biochemistry and Biotechnology162, 1574–1584.

20.   Frauenkron-Machedjou VJ, Fulton A, Zhao J, Lina Weber L, Jaeger KE, Schwaneberg U, Zhu L. (2018) Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance. Bioresources and Bioprocessing 5, 2.

21.   Kardashliev T, Ruff AJ, Zhao J, Schwaneberg U. (2014) A high-throughput screening method to reengineer DNA polymerases for random mutagenesis. Molecular Biotechnology 56, 274–283.

22.   曲戈, 赵晶, 郑平, 孙际宾, 孙周通*. 定向进化技术的最新进展. 生物工程学报, 2018, 34 (1): 1–11.

23.   赵晶, 鞠鑫, 潘江, 李春秀, 王敏杰, 许建和*. 毛白杨环氧水解酶的异源表达及其在催化拆分手性环氧化物中的应用. 催化学报, 2012, 33 (2): 302–307.