副教授

赵晶

发布时间:2019-08-01    点击率:

 

姓  :       赵晶

办公电话:     15522275019(手机)

电子邮件:      zhaojing@hubu.edu.cn, zhaojing802@hotmail.com

通讯地址:      湖北省武汉市武昌区友谊大道368号湖北大学生命科学学院

基本情况简介 博士,现任湖北大学副教授、硕士研究生导师

主要研究领域: 计算生物学酶工程和生物催化

学习和工作简历:

l 20049-20087月,南京农业大学,获工学学士学位

l 20089-20116月,华东理工大学,获工学硕士学位

l 201110-20159月,德国亚琛工业大学(RWTH Aachen University),获理学博士学位

l 201512-201812月,中国科学院天津工业生物技术研究所,助理研究员

l 20191-20197月,湖北大学生命科学学院,讲师

l 20197月至今,湖北大学生命科学学院,副教授

承担科研项目情况

1. 国家自然科学基金(21606251):赖氨酸脱羧酶多聚体界面氨基酸对酶活性和最适pH影响规律的研究;2017.01-2019.12,主持。

2. 天津市自然科学基金(18JCQNJC10300):手性砌块反式-4-羟基-L-脯氨酸生物法绿色制造关键酶的多目标分子进化与应用研究;2018.04-2021.03,主持。

3. 天津市留学人员科技活动启动项目择优资助(2017年度):同时进化酶的可溶性和活性获得新型赖氨酸脱羧酶的研究,2018.01-2018.12,主持。

4. 国家自然科学基金(31870081):以异亮氨酰-tRNA合成酶为例构建一类新型氨基酸生物传感器;2019.01-2022.12,参与。

 

代表性文章(*通讯作者):

1. Jing Zhao#, Chao Liu#, Xuan Guo#, Jinyu Wang, Haiping Liu, Ping Zheng*, Jibin Sun*, Yanhe Ma. Efficient production of trans-3-hydroxyproline by a bacterial trans-3-proline hydroxylase and characterization of enzymatic properties. Biochemical Engineering Journal, 2019, 147:57–61.

2. Chao Liu#, Jing Zhao# (#equal contribution), Jiao Liu, Xuan Guo, Deming Rao, Haiping Liu, Ping Zheng*, Jibin Sun*, Yanhe Ma. Simultaneously improving the activity and thermostability of a new proline 4-hydroxylase by loop grafting and site-directed mutagenesis, Applied Microbiology and Biotechnology, 2019, 103:265–277.

3. Jing Zhao, Victorine Josiane Frauenkron-Machedjou, Alexander Fulton, Leilei Zhu, Mehdi D. Davari, Karl-Erich Jaeger, Ulrich Schwaneberg*, Marco Bocola*, Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study, Physical Chemistry Chemical Physics, 2018, 20(14): 9600–9609.

4. Jing Zhao, Victorine Josiane Frauenkron-Machedjou, Tsvetan Kardashliev, Anna Joëlle Ruff, Leilei Zhu, Marco Bocola, Ulrich Schwaneberg*. Amino acid substitutions in random mutagenesis libraries: lessons from analyzing 3000 mutations. Applied Microbiology and Biotechnology, 2017, 101(8): 3177–3187.

5. Jing Zhao, Ning Jia, Karl-Erich Jaeger, Marco Bocola, Ulrich Schwaneberg*. Ionic liquid activated Bacillus Subtilis lipase A variants through cooperative surface substitutions. Biotechnology and Bioengineering, 2015, 112(10): 1997–2004.

6. Jing Zhao, Tsvetan Kardashliev, Anna Joëlle Ruff, Marco Bocola, Ulrich Schwaneberg*. Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations. Biotechnology and Bioengineering, 2014, 111(12): 2380–2389.

7. Jing Zhao, Yanyan Chu, Aitao Li, Xin Ju, Xudong Kong, Jiang Pan, Yun Tang*, Jianhe Xu*. An unusual (R)-selective epoxide hydrolase with high activity for facile preparation of enantiopure glycidyl ethers. Advanced Synthesis & Catalysis, 2011, 353 (9): 1510–1518.

8. Fengyu Kou#, Jing Zhao# (#equal contribution), Jiao Liu, Jie Shen, Qin Ye, Ping Zheng, Zhimin Li*, Jibin Sun*, Yanhe Ma. Characterization of a new lysine decarboxylase from Aliivibrio salmonicida for cadaverine production at alkaline pH. Journal of Molecular Catalysis B: Enzymatic, 2016, 133(Supplement 1): S88–S94.

9. Zijian Tan, Jing Zhao# (#equal contribution), Jiuzhou Chen, Deming Rao, Wenjuan Zhou, Ning Chen, Ping Zheng*, Jibin Sun, Yanhe Ma. Enhancing thermostability and removing hemin inhibition of Rhodopseudomonas palustris 5-aminolevulinic acid synthase by computer-aided rational design. Biotechnology Letters, 2019, 41:181–191.

10. Deyu Xu#, Jing Zhao# (#equal contribution), Guoqiang Cao, Jinyu Wang, Qinggang Li, Ping Zheng*, Shuxin Zhao, Jibin Sun. Removal of feedback inhibition of Corynebacterium glutamicum phosphoenolpyruvate carboxylase by addition of a short terminal peptide. Biotechnology and Bioprocess Engineering, 2018, 23(1): 72–78.

11. Jiao Liu, Yu Wang, Yujiao Lu, Xiaomeng Ni, Xuan Guo, Jing Zhao, Jiuzhou Chen, Taiwo Dele-Osibanjo, Ping Zheng, Jibin Sun*, Yanhe Ma. Mutations on peptidoglycan synthesis gene ponA improve electro-transformation efficiency of Corynebacterium glutamicum ATCC 13869, Applied and Environmental Microbiology, 2018, 84 (24) e02225-18.

12. Renjie Li, Aitao Li, Jing Zhao, Qi Chen, Ning Li, Huilei Yu*, Jianhe Xu. Engineering P450LaMO stereospecificity and product selectivity for selective C-H oxidation of tetralin-like alkylbenzenes. Catalysis Science & Technology, 2018, 8: 4638–4644.

13. Guochao Xu, Yue Wang, Minghui Tang, Jieyu Zhou, Jing Zhao, Ruizhi Han, Ye Ni*. Hydroclassified combinatorial saturation mutagenesis: reshaping substrate binding pockets of KpADH for enantioselective reduction of bulky-bulky ketones. ACS Catalysis, 2018, 8: 8336–8345.

14. Renjie Li, Jianhe Xu*, Qi Chen, Jing Zhao, Aitao Li, Huilei Yu*, Enhancing the catalytic performance of a CYP116B monooxygenase by transdomain combination mutagenesis. ChemCatChem, 2018, 10(14): 2962–2968.

15. Xingchu Wang#, Jiao Liu#, Jing Zhao, Xiaomeng Ni, Ping Zheng, Xuan Guo, Cunmin Sun, Jibin Sun*, Yanhe Ma, Efficient production of trans-4-hydroxy-L-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. Journal of Bioscience and Bioengineering, 2018, 126(4): 470–477.

16. Fengyu Kou, Jing Zhao, Jiao Liu, Cunmin Sun, Yanmei Guo, Zijian Tan, Feng Cheng, Zhimin Li, Ping Zheng*, Jibin Sun, Enhancement of the thermal and alkaline pH stability of Escherichia coli lysine decarboxylase for efficient cadaverine production. Biotechnology Letters, 2018, 40(4): 719–727.

17. Ulrich Markel, Leilei Zhu, Victorine Josiane Frauenkron-Machedjou, Jing Zhao, Marco Bocola, Mehdi D. Davari, Karl-Erich Jaeger, Ulrich Schwaneberg*. Are directed evolution approaches efficient in exploring nature’s potential to stabilize a lipase in organic cosolvents? Catalysts, 2017, 7(5): 142.

18. Hao Cao, Chengcheng Li, Jing Zhao, Fang Wang, Tianwei Tan, Luo Liu*. Enzymatic production of glutathione coupling with an ATP regeneration system based on polyphosphate kinase. Applied Biochemistry and Biotechnology, 2018, 185(2): 385–395.

19. Xiang Liu, Jianhe Xu*, Jiang Pan, Jing Zhao. Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01. Applied Biochemistry and Biotechnology, 2010, 162: 1574 –1584.

20. Victorine Josiane Frauenkron-Machedjou, Alexander Fulton, Jing Zhao, Lina Weber, Karl-Erich Jaeger, Ulrich Schwaneberg, Leilei Zhu*. Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance. Bioresources and Bioprocessing, 2018, 5: 2.

21. Tsvetan Kardashliev, Anna Joëlle Ruff, Jing Zhao, Ulrich Schwaneberg*. A high-throughput screening method to reengineer DNA polymerases for random mutagenesis. Molecular Biotechnology, 2014, 56(3): 274–283.

22. 曲戈, 赵晶, 郑平, 孙际宾, 孙周通*. 定向进化技术的最新进展. 生物工程学报, 2018, 34 (1): 111.

23. 赵晶, 鞠鑫, 潘江, 李春秀, 王敏杰, 许建和*. 毛白杨环氧水解酶的异源表达及其在催化拆分手性环氧化物中的应用. 催化学报, 2012, 33 (2): 302307.

 

 

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