教授

李爱涛

发布时间:2018-10-23    点击率:

 

 

: 李爱涛

办公电话: 027-8866123718871879016(手机);

电子邮件: aitaoli@hubu.edu.cn,aitaoli@163.com

通讯地址: 湖北省武汉市武昌区友谊大道368号,湖北大学生命科学学院

任职情况:博士,现任湖北大学教授、博士研究生导师

一、学习和工作简历

l 20019-20057月,河北工业大学,生物工程,本科

l 20059-20108月,华东理工大学,发酵工程,博士

l 20112-20145月,新加坡国立大学,博士后研究员

l 20149-20179月,德国煤炭研究所/马尔堡大学,博士后研究员

l 20179-今,博士研究生导师,教授

二、承担科研项目情况

1. 国家自然科学基金(21702052):基于P450酶定向羟化的去甲(伪)麻黄碱对映体新合成途径研究, 经费:26万元;2018,1-2020,12,主持人。

2. 生物反应器工程国家重点实验室开放课题:7β-甾体羟化酶分子改造及其催化机制研究;经费:10万元;2018,8-2020,7,主持人。

三、代表性论文(*通讯作者)

1. Ai-Tao Li, Carlos G. Acevedo-Rocha, Manfred T. Reetz. Boosting the Efficiency of Site-Saturation Mutagenesis for a Difficult-to-Randomize Gene by a Two-Step PCR Strategy. Applied Microbiology and Biotechnology. 2018, 102, 6095-6103 (二区 top)

2. Wenwen She, Jing Ni, Ke Shui, Fei Wang, Ruyi He, Jinhui Xue, Manfred T. Reetz, Aitao Li*, Lixin Ma*. Rapid and error-free site-directed mutagenesis by a PCR-free in vitro CRISPR/Cas9-mediated mutagenic system. ACS Synthetic Biology 2018, (accepted) (通讯作者). (二区 top)

3. Ai-Tao Li, Carlos G. Acevedo-Rocha, Zhoutong Sun, Tony Cox, Jia (Lucy) Xu, and Manfred T. Reetz*. Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. ChemBioChem 201819 (3), 221-228 (被选为杂志封面). (三区)

4. Ai-Tao Li, zhoutong Sun, MT Reetz. Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution? ChemBioChem 2018 (accepted, 三区)

5. Ai-Tao Li, Binju Wang, Adriana Ilie, Kshatresh D. Dubey, Gert Bange, Ivan V. Korendovych, Sason Shaik, Manfred T. Reetz. A redox-mediated Kemp eliminase. Nature Communications, 2017, 8, 14876. (一区 top)

6. Ai-Tao Li, Adriana Ilie, Zhoutong Sun, Richard Lonsdale, Jian-He Xu, Manfred T. Reetz. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. Angewandte Chemie International Edition. 2016, 128,12205-12208. (一区top)

7. Yucai He*, Yun Ding , Cuiluan Ma , Junhua Di , Chunxia Jiang and Ai-Tao Li*. One-pot conversion of biomass-derived xylose to furfuralcohol by a chemo-enzymatic sequential acid-catalyzed dehydration and bioreduction. Green Chemistry, 2017, 19 (16), 3844-3850  (一区 top)

8. Ai-Tao Li, Shuke Wu, Joseph P Adams, Radka Snajdrova, Zhi Li. Asymmetric Epoxidation of Alkene and Benzylic Hydroxylation with P450tol from Rhodococcus coprophilus TC-2. Chemical Communications, 2014, 50, 8771-8774.  (higlighted in synfacts) (一区top)

9. Ai-Tao Li, Shuke Wu, Joseph P Adams, Radka Snajdrova, Zhi Li Asymmetric Oxidation with P450tol Monooxygenase. Synfacts, 2014, 10: 1097-1097.

10. Ai-Tao Li, Ji Liu, Son Q. Pham, Zhi Li. Engineered P450pyr monooxygenase for asymmetric epoxidation of alkenes with unique and high enantioselectivity. Chemical Communications, 2013, 49, 11572-11574. (一区 top)

11. HL Yu, T Li, FF Chen, XJ Luo, Ai-Tao Li, C Yang, GW Zheng, JH Xu. Bioamination of alkane with ammonium by an artificially designed multienzyme cascade. Metabolic engineering 2018, 47, 184-189. (一区)

12. Ren-Jie Li, Ai-Tao Li, Jing Zhao, Qi Chen, Ning Li, Huilei Yu, Jian-He XuEngineering P450LaMO Stereospecificity and Product Selectivity for Selective CH Oxidation of Tetralin-like Alkylbenzenes. Catalysis Science & Technology 2018, (accepted, 二区)

13. Ren-Jie Li, Jian-He Xu, Qi Chen, Jing Zhao, Ai-Tao Li, HuiLei Yu. Enhancing the catalytic performance of a CYP116B monooxygenase by transdomain combination mutagenesis. ChemCatChem 2018, (accepted二区)

14. Carlos G Acevedo-Rocha, Charles G Gamble, Richard Lonsdale, Ai-Tao Li, Nathalie Nett, Sabrina Hoebenreich, Julia B Lingnau, Cornelia Wirtz, Christophe Fares, Heike Hinrichs, Alfred Deege, Adrian J Mulholland, Yuval Nov, David Leys, Kirsty J McLean, Andrew W Munro, Manfred T Reetz. P450-Catalyzed Regio-and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping. ACS Catalysis 2018, 8, 3395-3410. (一区)

15. Ai-Tao Li, TPN Ngo, Jingyong Yan, Kaiyuan Tian, Zhi Li. Whole-cell based solvent-free system for one-pot production of biodiesel from waste grease. Bioresource Technology, 2012, 114: 725-729. (一区)

16. Ai-Tao Li, Hui-Lei Yu, Jiang Pan, Jian-Dong Zhang, Jian-He Xu, Guo-Qiang Lin. Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides. Bioresource Technology, 2011, 102: 1537-1542. (一区)

17. Ai-Tao Li, Jian-Dong Zhang, Hui-Lei Yu, Jiang Pan, Jian-He Xu. Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system. Process Biochemistry, 2011, 46: 689-694. (三区)

18. Ai-Tao Li, Jian-He Xu, Guo-Qiang Lin. Isolation of Rhodococcus sp. ECU0066: A new sulfide monooxygenase producing strain for asymmetric sulfoxidation. Applied and Environmental Microbiology, 2009, 75: 551-556. (二区)

外文专著:

1. Ai-Tao Li, Zhi, Li. Asymmetric synthesis of enantiopure epoxideds using monooxgenases. in Science of Synthesis: Biocatalysis in Organic Synthesis 2. ed, Faber, K., Fessner, W.-D., Turner, N.J. Thieme, Stuttgart, Germany, 2015

2. Ai-Tao Li, Gao-Wei Zheng, Hui-Lei Yu, Jiang Pan, Chun-Xiu Li, Jian-He Xu. Preparation of Enantiopure Sulfoxides by Enantioselective Oxidation with Whole Cells of Rhodococcus sp. ECU0066. Practical Methods for Biocatalysis and Biotransformations 2. Eds., John Whittall and Peter W. Sutton John Wiley & Sons, 2012

四、专利

1. 许建和、李爱涛、张建栋、卢文芽、林国强。一株红球菌以及该菌株制备光学纯手性亚砜的用途中国专利号:ZL200810042425.3.

2. Zhi Li, Nguyen Phuong Thao Ngo, Wen Wang, Zillillah, Guowei Tan, Ai-Tao Li, Jin-Yong Yan, Method of converting grease containing high content of free fatty acids to fatty acid esters and catalysts for use in said method. WO Patent, 2013, 2013048346.

五、获奖

1. 2013 Best poster award, GSM Symposium, 新加坡国立大学

2. 2012 优秀博士论文奖, 华东理工大学

3. 2010 杰出研究成果奖, 华东理工大学

六、招贤纳士

1. 本实验室招博后、讲师及副教授。有分子生物学、酶学、蛋白质工程、有机化学背景,发表过SCI论文,具有合作精神。可将简历发送至aitaoli@hubu.edu.cn,待遇面议。

2. 本实验室长期招收有探索精神、动手能力强并踏实细致的本科生,有机化学功底强者优先。

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