教授

易犁

发布时间:2015-07-16    点击率:

 

姓  名: 易犁

办公电话: 027-88661237

电子邮件: liyi@hubu.edu.cn or li_yi@hotmail.com

通讯地址: 湖北省武汉市武昌区友谊大道368号湖北大学生命科学学院

基本情况简介:博士,现任湖北大学教授、博士研究生导师

简介:美国密歇根大学生物化学博士;回国前任职美国德州大学奥斯汀分校及克雷登研究基金会副研究员、研究员职位;曾获美国密歇根大学Anthony and Lillian Lu Award;现为湖北大学教授,博士生导师,湖北省第六批百人计划特聘教授,湖北省生物工程学会理事,生物催化技术湖北省工程实验室副主任,中国抗生素杂志编委会委员,武汉3551光谷人才计划;已获得美国授权专利1项、欧盟专利申请1项、加拿大专利申请1项、中国专利申请9项,发表包括PNAS, NAR, JBC, ACS Chem Biol在内的高水平SCI论文30余篇。

主要研究领域:分子酶工程(蛋白酶/抗体)、蛋白表达和高通量技术、系统生物学、生物传感

1) Protease/Antibody Engineering for Industrial and Therapeutic Applications: focusing on protease engineering for its applications in industry and medicine (acute autoimmune diseases), scFV and Fab engineering of anti-TNF-α and anti-MMPs antibodies for therapeutics (Yeast surface/Phage display technologies, Structure modeling, Directed evolution,High-throughput screening and sequencing, Biocatalysis, Mass Spectrometry, Enzyme kinetics, Machine Learning, etc.)

2) Protein Folding & Secretion Mechanisms in Eukaryotic Cells: exploring the protein folding and secretion mechanisms in eukaryotic cells, in-depth analysis of protein interactions and modifications in yeast cells, and ER disorder associated human diseases (Systematic biology, Biochemistry, PPI, High-throughput sequencing, Bioinformatics, etc.)

3) Proteins/Chemicals Production in Microbial Systems:developing efficient microbial cell factories (S. cerevisiae, P. pastoris, E. coli, B. subtilis, etc) using systems biology, synthetic biology, and metabolic engineering for proteins and chemicals production (Strain engineering, Protein folding, Secretion, and Cell surface immobilization, etc.)

学习和工作简历:

2015 - ,湖北大学生命科学学院教授,博士生导师

2010 -2015,美国德州大学奥斯汀分校& Clayton研究基金会,博士后,副研究员(Research Associate),研究员(Research Fellow) (导师:Prof. George Georgiou & Prof. Brent L. Iverson– 美国工程学院院士、美国医学院院士、美国科学促进协会院士)

2010,美国密歇根大学安娜堡分校,生物化学博士学位 (导师:Prof. Stephen W. Ragsdale ­–美国科学促进协会院士、美国微生物协会院士)

2003,华中农业大学,微生物学硕士学位 (导师:周启教授,周俊初教授,胡传炯教授)

2000年,华中农业大学,微生物学学士学位

主持科研项目情况:

1) 国家重点研发计划课题(科技部,2018YFA090091-03),高灵敏环境持久性有毒污染物感知与识别生物系统,2019-2024150万,子课题主持人

2) 2018年国家自然科学基金(科技部,国家自然科学基金委,31870057 ):酿酒酵母内质网滞留信号介导的蛋白滞留效应研究, 2019-202259万,主持人

3) 国家重点研发计划课题(2018YFD0500200-03),畜禽废弃物昆虫高效转化机制与调控研究,2018-202028万,子课题主持人

4) 2018年针对湖泊水样及周边土壤重金属吸附微生物快速分离、鉴定技术开发,(横向课题,安徽锦域生物科技有限公司),2018-201910万,主持人;

5) 2018年毕赤酵母表达鳗鲡生长激素(EelGH)高产菌株技术开发,(横向课题,武汉派锐生物研发有限公司),2018-202020万,主持人;

6) 2017PK15抑制圆环病毒复制相关基因敲除细胞系构建,(横向课题,浙江美保龙生物技术有限公司),2017-202030万,主持人;

7) 2016年国家自然科学基金(国家自然科学基金委,31540068 ):酵母内质网内蛋白滞留效应及蛋白酶介导的翻译后修饰研究, 16万,主持人;

8) 2015年湖北省自然科学基金重点项目(湖北省科技厅,2015CFA088 ):酵母内质网全蛋白酶图谱的绘制及提高人源溶栓蛋白酶表达的研究,2016-201810万,主持人;

9) 2016年湖北省百人计划资助基金,(湖北省组织部,湖北大学),100万,主持人;

10) 2015年生物资源绿色转化湖北省协同创新中心人才引进课题资助(湖北省科技厅):针对具有工业医用效应酶的分子改造及特性研究,400万,主持人;

选择发表论文:

1) Mei, M., Li, J., Wang, S., Lee, B., Iverson B. L., Zhang, G., Ge, X.,* and Yi, L. *," Functional Fab yeast surface display of Adalimumab and its variants against TNF-α through a bi-directional promoter strategy", Front. Bioeng. Biotechnol., 2019

2) Fan, X., Li, X., Zhou, Y., Mei, M., Peng, W., Jiang, Z., Yang, S., Iverson B. L., Zhang, G., * and Yi, L. *,"Quantitative analysis of substrate specificities of the 3C protease of human rhinovirus and the exploration of its substrate recognition mechanisms ", ACS Chem. Biol., 2019

3) Zheng, Y., Han, J., Wang, B., Hu, X., Li, R., Shen, W., Ma, X., Ma, L., Yi, L.*, Yang, S.*, and Peng, W.*," Characterization and repurposing of the endogenous Type I-F CRISPR-Cas system of Zymomonasmobilis for genome engineering ", Nucleic Acids Res., 2019

4) Yang, Y., Shen, W., Li, R., Huang, J., Xiao, Y., Wei, H., Chou, Y., Zhang, M., Himmel, M. E., Chen, S., Yi, L.,Ma, L., and Yang, S.*, “Application of Zymomonasmobilis in biotechnology based on tools from the system biology era”, Biotechnol. Biofuels, 2019 Mar 14;12:52

5) Lu, Z., Yang, S., Yuan, X., Shi, Y., Ou, Y., Jiang, S., Yi, L.,and Zhang, G.*, "CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis ", 2019, Nucleic Acids Res., 2019 Apr 23;47(7):e40.

6) Mei, M., Zhai, C., Li, X. Z., Zhou, Y., Peng, W. F., Ma, L., Wang, Q. H., Iverson B. L., Zhang, G. M. *, and Yi, L.* “Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum of Saccharomyces cerevisiae”, J. Biol. Chem., 2017, Dec 15; 292(50):20707-20719

 

7) Mei, M., Zhou, Y., Peng W. F., Yu, C., Ma, L. X., Zhang, G. M.*, and Yi, L.* “Application of Modified Yeast Surface Display Technologies for Non-Antibody Protein Engineering”, Microbiol. Res., 2017 Mar;196:118-128

8) Li, Q.,#Yi, L.,# Hoi, K. H., Marek, P., Georgiou, G. *, and Iverson, B. L.* “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing”, ACS Chem. Biol., 2017 Feb 17;12(2):510-518. (#: co-first author.)

9) Song, H. T.,Gao, Y., Yang, Y. M., Xiao, W. J., Liu, S. H., Xia, W. C., Liu, Z. L., Yi, L., and Jiang, Z. B.* “Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates”, Bioresour. Technol., 2016, 219:710-5

10) Yi, L. *,Li, Q., Taft, J. M., Gebhard, M. C., Iverson, B. L. *, and Georgiou, G. * “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast”, Methods Mol. Biol. 2015, 1319:81-93.

11) Li, Q., Yi, L., and Marek, P. “Antibodies and Their Multivalent Constructs for Cancer Therapy”, Protein Pept. Lett. 2014, 21(10):1017-24

12) Yi, L.,Gebhard, M. C., Li, Q., Taft, J. M., Georgiou, G., and Iverson, B. L. “Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatiorial libraries”, PNAS, 2013, 110(18):7229-34.

13) Ragsdale, S. W., Yi, L., et al. “Redox, Haem and CO in Enzymatic Catalysis and Regulation”, Biochem. Soc. Trans., 2012, 40(3):501-7.

14) Ragsdale, S. W., and Yi, L. “Thiol/Disulfide Redox Switches in The Regulation of Heme Binding to Proteins”, Antioxid. Redox Signal., 2011, 14(6):1039-1047.

15) Yi, L., Morgan, J. T., and Ragsdale, S. W. “Identification Of a Thiol/Disulfide Redox Switch in The Human BK Channel That Controls Its Affinity For Heme And CO”, J. Biol. Chem., 2010, 285(26):20117-20127.

16) Yi, L., Jenkins, P. M., Leichert, L. I., Jakob, U., Martens, J. R., and Ragsdale, S. W. “Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to The Cellular Redox State” J. Biol. Chem., 2009, 284, 20556-20561.

17) Bianchetti, C. M., Yi, L.,Ragsdale, S. W., and Philips, G. N., Jr."Comparison Of Apo- And Heme-Bound Crystal Structures Of a Truncated Human Heme Oxygenase-2", J. Biol. Chem.,2007, 282, 37624-37631.

18) Yi, L., and Ragsdale, S. W. "Evidence That The Heme Regulatory Motifs in Heme Oxygenase-2 Serve As a Thiol/Disulfide Redox Switch Regulating Heme Binding", J. Biol. Chem., 2007, 282, 20156-21067.

:本实验室长期招收有探索精神、踏实认真的硕士与博士研究生,请邮件联系liyi@hubu.edu.cn

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