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  • 易犁
  • 作者:系统管理员    发布日期:2015-07-16    点击率:
  • 姓  名: 易 犁
    办公电话: 027-88661237-8039;
    电子邮件: liyi@hubu.edu.cn or li_yi@hotmail.com 
    通讯地址: 湖北省武汉市武昌区友谊大道368号
    湖北大学生命科学学院, 分子酶工程团队
    基本情况简介:博士,现任湖北大学教授,博士生导师 
    主要社会兼职:湖北省第六批百人计划特聘教授, 
                  湖北省生物工程学会理事, 
                  生物催化技术湖北省工程实验室副主任
    主要研究领域:酶工程,分子进化,系统免疫学,酶的化学生物学,和高通量筛选技术
     
    学习和工作简历:
    1996年9月-2000年7月,华中农业大学,微生物学学士学位
    2000年9月-2003年7月,华中农业大学,微生物学硕士学位 (周俊初,周启,胡传炯)
    2004年8月-2007年7月,美国内布拉斯加大学,生物化学硕士学位 (Dr. Stephen W. Ragsdale’s Lab)
    2007年8月-2010年4月,美国密歇根大学安娜堡分校,生物化学博士学位 (Dr. Stephen W. Ragsdale’s Lab)
    2010年11月-2012年5月,美国德州大学奥斯汀分校,博士后(Drs. George Georgiou & Brent L. Iverson’s Lab)
    2012年6月-2014年1月,美国德州大学奥斯汀分校 & Clayton研究基金会,副研究员(Research Associate)
    2014年2月-2014年8月,美国德州大学奥斯汀分校 & Clayton研究基金会,研究员(Research Fellow)
    2014年9月-今,湖北大学生命科学学院教授,博士生导师
     
    承担科研项目情况:
    1. 2016年湖北省百人计划资助基金,(湖北省组织部,湖北大学),主持人; 
    2. 2016年国家自然科学基金应急项目 (国家自然科学基金委,31540068 ):酵母内质网内蛋白滞留效应及蛋白酶介导的翻译后修饰研究, 2016,1-2016,12,主持人; 
    3. 2015年湖北省自然科学基金重点项目(湖北省科技厅,2015CFA088 ):酵母内质网全蛋白酶图谱的绘制及提高人源溶栓蛋白酶表达的研究2015,1-2017,12,主持人;
    4. 生物资源绿色转化湖北省协同创新中心人才引进课题资助(湖北省科技厅):针对具有工业医用效应酶的分子改造及特性研究2014.09-2018.12,主持人;
     
    代表性论文及专利(*通讯作者):
    2014-至今(湖北大学) 
    1. Mei, M., Zhou, Y., Peng, W. F., Yu, C., Zhai, C., Iverson B. L., Zhang, G. M*., and Yi, L.* “The Role of Amino Acid at Positions -5 and -6 of FEHDEL Determining the Endoplasmic Reticulum Retentional Effect Within the Saccharomyces cerevisiae”, 2016, Submitted 
    2. Mei, M., Zhou, Y., Peng W. F., Yu, C., Ma, L. X., Zhang, G. M*., and Yi, L.* “Application of Modified Yeast Surface Display Technologies for Non-Antibody Protein Engineering”, 2016, Submitted 
    3. Yi, L.,# Li, Q.,# Hoi, K. H., Georgiou, G., and Iverson, B. L., “Mapping the Endogenous Protease CleaveOme in the Yeast Secretory Pathway and Recombinant Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing”, 2016, Submitted (#: co-1st author)
    4. Ma, X., Liu, Y., Li, Q., Liu, L., Yi, L., Ma, L., and Zhai, C.* “Expression, Purification and Identification of a Thermolysin-like Protease, Neutral Protease I, From Aspergillus oryzae With the Pichia pastoris Expression System”, Protein Expr. Purif., 2016, 128:52-59    
    5. Song, H. T., Gao, Y., Yang, Y. M., Xiao, W. J., Liu, S. H., Xia, W. C., Liu, Z. L., Yi, L., and Jiang, Z. B.* “Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates”, Bioresour. Technol., 2016, 219:710-5 
    6. Zhang, Z., Tang, R., Bian, L., Mei, M., Li, C., Ma, X., Yi, L.*, and Ma, L. * “Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli”, PLoS One. 2016, 11(8):e0160367.
    7. Zhang, L., Mei, M., Yu, C., Shen, W. W., Ma, L., He, J. W., and Yi, L.* “Effector Proteins of Yersinia in Cell Infection”, Polish Journal of Microbiology, 2016, 65(1):5-12
    8. Li, H., He, R. Y., Xiong, X. M., Zhang, M. C., Yang, T. Y., Jiang, Z. B., Ma, L. Ma, X. D. *, and Yi, L.*  “Dynamic Diversification of bacterial Functional Groups in The Baiyunbian Liquor Stacking Fermentation Process”, Annals of Microbiology, 2016, DOI: 10.1007/s13213-016-1211-9
    9. Cai, H., Zhai, X. L., Mei, M., Zhou, Y., Zhang, L. L., Shen, W. W., Ma, L. X., He, J. W., and Yi, L.*  “Dithiolopyrrolones: Biosynthesis, Broad-spectrum Activities and Application”, 2016, Chinese J. Antibiot., 2016, 41(6):401-410
    10. Zhang, X., Liu, J., Wang, F., Yi, L., Li, Z., Liu, Y., anf Ma, L. “High-level Expression of Human Arginase I in Pichia pastoris and Its Immobilization on Chitosan to Produce L-ornithine”, BMC Biotechnol., 2015, July 31; 15:66 
    11. Li, Q., Yi, L., and Marek, P. “Antibodies and Their Multivalent Constructs for Cancer Therapy”, Protein & Peptide Lett. 2014, 21(10):1017-24
    专利:
    1. 一种滞留效应增强的内质网滞留信号肽突变体;易犁,梅萌,张桂敏,马立新,马延和;专利申请号:201510658688.7
    2. 一种不同启动子介导的蛋白酶高通量筛选方法;易犁,周瑜,张桂敏,彭文舫,马立新,马延和;专利申请号:201510802312.9
     
    2007-2015(University of Nebraska, University of Michigan, University of Texas at Austin, Clayton Research Foundation) 
    1. Yi, L.,* Li, Q., Taft, J. M., Gebhard, M. C., Iverson, B. L., and Georgiou, G. “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast”, Methods Mol. Biol. 2015, 1319:81-93.
    2. Yi, L.,Gebhard, M. C., Li, Q., Taft, J. M., Georgiou, G., and Iverson, B. L. “Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatiorial libraries”, PNAS, 2013, 110(18):7229-34.
    3. Li, Q., Yi, L.,Marek, P., and Iverson, B. L. “Commercial proteases: present and future.”,FEBS Lett. 2013, 587(8):1155-63.
    4. Ragsdale, S. W., Yi, L., et al. “Redox, Haem and CO in Enzymatic Catalysis and Regulation”, Biochem. Soc. Trans.,2012, 40(3):501-7.  
    5. Ragsdale, S. W.,and Yi, L. “Thiol/Disulfide Redox Switches in The Regulation of Heme Binding to Proteins”, Antioxid. Redox Signal.,2011, 14(6):1039-1047. 
    6. Yi, L., Morgan, J. T., and Ragsdale, S. W. “Identification Of a Thiol/Disulfide Redox Switch in The Human BK Channel That Controls Its Affinity For Heme And CO”, J. Biol. Chem., 2010, 285(26):20117-20127. 
    7. Gardner, J. D., Yi, L., Ragsdale, S. W., and Brunold, T. C. “Spectroscopic Insights Into Axial Ligation And Active-Site H-Bonding in Substrate-Bound Human Heme Oxygenase-2”, J. Biol. Inorg. Chem., 2010, 15(7):1117-1127. 
    8. Yi, L., Jenkins, P. M., Leichert, L. I., Jakob, U., Martens, J. R., and Ragsdale, S. W. “Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to The Cellular Redox State” J. Biol. Chem., 2009, 284, 20556-20561. 
    9. Bianchetti, C. M., Yi, L.,Ragsdale, S. W., and Philips, G. N., Jr."Comparison Of Apo- And Heme-Bound Crystal Structures Of a Truncated Human Heme Oxygenase-2", J. Biol. Chem.,2007, 282, 37624-37631. 
    10. Yi, L., and Ragsdale, S. W. "Evidence That The Heme Regulatory Motifs in Heme Oxygenase-2 Serve As a Thiol/Disulfide Redox Switch Regulating Heme Binding", J. Biol. Chem., 2007, 282, 20156-21067. 
    专利:
    1. Method for Engineering Proteases and Protein Kinases. Brent L. Iverson, George Georgiou, Joseph M. Taft, and Li Yi, 2014, 
    -US Patent NO., 8,945,855;  
    - Canadian Patent Application NO., 2,877,346; 
    - European Patent Application NO., 13734622.7
     
    2007之前(华中农业大学): 
    1. Yi, L., Wang, W., Li, M. Y., and Liu H. Z., “A Study Of The Geographical Differentiation Of RhodeusOcellatusBased On RAPD Analysis”,Acta. Hydrobiol. Sin., 2001, 25(3):301-304. 
     
    获奖:
    1. Rackham Travel Award, University of Michigan at Ann Arbor, 2010
    2. Anthony and Lillian Lu Award, University of Michigan at Ann Arbor, 2009
    3. Rackham Travel Award, University of Michigan at Ann Arbor, 2009
     
    国际会议特邀报告:
    1. “Yeast ER Retention Effect, An Old Physiological Nature Utilized In New Protein Engineering”, Section of Genetics of Yeasts, the 13th International Symposium on the Genetics of Industrial Microorganisms, 2016, Wuhan, China
    2. “YESS: A New Protease Engineering Platform”, Section of Advances in Biocatalysis, 246th National Annual Meeting of American Chemical Society, 2013, Indianapolis, IN, USA
     
    国内会议特邀报告:
    1. 2015年第八届中国工业生物技术发展高峰论坛
    2. 2014年湘鄂微生物学基础与应用学术研讨会
    3. 2014年湖北省生物工程学会学术年会

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